Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1983 Jan 01;801:51-5. doi: 10.1073/pnas.80.1.51.
Show Gene links
Show Anatomy links
Structural features of a phased nucleosome core particle.
Simpson RT
,
Stafford DW
.
???displayArticle.abstract???
Chicken erythrocyte inner histones associate with a cloned 260-base-pair (bp) segment of Lytechinus variegatus DNA in a unique location. The fragment contains a 120-bp segment encoding 5S rRNA, a 90-bp flanking sequence to the 5'' side of the transcribed segment, and a 50-bp downstream flanking sequence. Association of DNA, uniquely labeled at one end or the other and at either the 3'' or the 5'' terminus of a given strand, with histones at 0.1 M ionic strength leads to formation of a compact complex which sediments at about 13 S. Analysis of cutting of the complex by DNase I shows that protection from the nuclease is confined to a region beginning 20 bp from the left end of the segment and extending to about 165 bp from the left end. Within the protected region, the two DNA strands differ in their susceptibilities to the nuclease, the precise location of nuclease cutting sites and the spacing between these sites, and the relative susceptibilities of specific cutting locations. It seems that information present in DNA and the histone octomer is sufficient to create a precisely phased nucleosome in which interactions of the two DNA strands with histones are not the same. The structure of this unique nucleosome is not predicted by the intellectual model based on studies of mixed populations of nucleosome core particles.
Bryan,
Core nucleosomes by digestion of reconstructed histone-DNA complexes.
1979, Pubmed
Bryan,
Core nucleosomes by digestion of reconstructed histone-DNA complexes.
1979,
Pubmed
Chao,
DNA sequence directs placement of histone cores on restriction fragments during nucleosome formation.
1979,
Pubmed
Crick,
Linking numbers and nucleosomes.
1976,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
Finch,
X-ray diffraction study of a new crystal form of the nucleosome core showing higher resolution.
1981,
Pubmed
Finch,
Structure of nucleosome core particles of chromatin.
1977,
Pubmed
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
Gottesfeld,
Nonrandom alignment of nucleosomes on 5S RNA genes of X. laevis.
1980,
Pubmed
Igo-Kemenes,
Chromatin.
1982,
Pubmed
Jakobovits,
A nucleosome-free region in SV40 minichromosomes.
1980,
Pubmed
Klug,
The helical periodicity of DNA on the nucleosome.
1981,
Pubmed
Louis,
Chromatin structure of the 5S RNA genes of D. melanogaster.
1980,
Pubmed
Lu,
Nucleotide sequence of a 5S ribosomal RNA gene in the sea urchin Lytechinus variegatus.
1980,
Pubmed
,
Echinobase
Lutter,
Precise location of DNase I cutting sites in the nucleosome core determined by high resolution gel electrophoresis.
1979,
Pubmed
Lutter,
DNase II digestion of the nucleosome core: precise locations and relative exposures of sites.
1981,
Pubmed
Maizel,
Enhanced graphic matrix analysis of nucleic acid and protein sequences.
1981,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Noll,
DNA folding in the nucleosome.
1977,
Pubmed
Olins,
Conformational states of chromatin nu bodies induced by urea.
1977,
Pubmed
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
Saragosti,
Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
1980,
Pubmed
Simpson,
Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends.
1976,
Pubmed
Simpson,
Random protection of single cut restriction endonuclease sites in SV40 mini-chromosomes assembled in vitro.
1980,
Pubmed
Simpson,
Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
1978,
Pubmed
Simpson,
Cromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence.
1979,
Pubmed
Sollner-Webb,
Pancreatic DNAase cleavage sites in nuclei.
1977,
Pubmed
Stein,
Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.
1979,
Pubmed
Trifonov,
Noninteger pitch and nuclease sensitivity of chromatin DNA.
1979,
Pubmed
Wasylyk,
Preferential in vitro assembly of nucleosome cores on some AT-rich regions of SV40 DNA.
1979,
Pubmed
