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Oxid Med Cell Longev
2018 Apr 19;2018:2087373. doi: 10.1155/2018/2087373.
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Anti-Inflammatory Activity of Marine Ovothiol A in an In Vitro Model of Endothelial Dysfunction Induced by Hyperglycemia.
Castellano I
,
Di Tomo P
,
Di Pietro N
,
Mandatori D
,
Pipino C
,
Formoso G
,
Napolitano A
,
Palumbo A
,
Pandolfi A
.
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Chronic hyperglycemia is associated with oxidative stress and vascular inflammation, both leading to endothelial dysfunction and cardiovascular disease that can be weakened by antioxidant/anti-inflammatory molecules in both healthy and diabetic subjects. Among natural molecules, ovothiol A, produced in sea urchin eggs to protect eggs/embryos from the oxidative burst at fertilization and during development, has been receiving increasing interest for its use as an antioxidant. Here, we evaluated the potential antioxidative/anti-inflammatory effect of purified ovothiol A in an in vitro cellular model of hyperglycemia-induced endothelial dysfunction employing human umbilical vein endothelial cells (HUVECs) from women affected by gestational diabetes (GD) and from healthy mothers. Ovothiol A was rapidly taken up by both cellular systems, resulting in increased glutathione values in GD-HUVECs, likely due to the formation of reduced ovothiol A. In tumor necrosis factor-α-stimulated cells, ovothiol A induced a downregulation of adhesion molecule expression and decrease in monocyte-HUVEC interaction. This was associated with a reduction in reactive oxygen and nitrogen species and an increase in nitric oxide bioavailability. These results point to the potential antiatherogenic properties of the natural antioxidant ovothiol A and support its therapeutic potential in pathologies related to cardiovascular diseases associated with oxidative/inflammatory stress and endothelial dysfunction.
Figure 2. Ovothiol A levels in endothelial cells and in culture media. HPLC profiles of cytosolic extracts of C-HUVECs (a) and GD-HUVECs (b) with and without treatment with ovothiol A (50 μM). Red line: C- and GD-HUVECs treated with ovothiol A for 10 min. Blue line: untreated C- and GD-HUVECs after 10 min. Detection: A280 nm. (c) Percentage of ovothiol A in the medium of C- and GD-HUVEC-treated cells.
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