Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Alpha-helix in the carboxy-terminal domains of histones H1 and H5.
Clark DJ
,
Hill CS
,
Martin SR
,
Thomas JO
.
???displayArticle.abstract???
Although the carboxy-terminal domains of histones H1 and H5 exist as random-coil in aqueous solution, secondary structure prediction suggests that this region has a high potential for alpha-helix formation. We have measured CD spectra in various conditions known to stabilize alpha-helices, to determine whether this potential can be realized in an appropriate environment. Trifluoroethanol increases the helix contents of H1, H5 and their carboxy-terminal fragments, presumably through promotion of axial hydrogen bonding. Sodium perchlorate is also effective and better than sodium chloride, suggesting stabilization by binding of bulky perchlorate ions rather than simple charge screening. Extrapolating from these measurements in solution, and taking into account the occurrence of proline residues throughout the carboxy-terminal domain, we propose that binding to DNA stabilizes helical segments in the carboxy-terminal domains of histones H1 and H5, and that it is this structured form of the domain that is functionally important in chromatin.
Allan,
Roles of H1 domains in determining higher order chromatin structure and H1 location.
1986, Pubmed
Allan,
Roles of H1 domains in determining higher order chromatin structure and H1 location.
1986,
Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Aviles,
The conformation of histone H5. Isolation and characterisation of the globular segment.
1978,
Pubmed
Banks,
Sequence, structure and activity of phosphoglycerate kinase: a possible hinge-bending enzyme.
1979,
Pubmed
Barbero,
Structural studies on histones H1. Circular dichroism and difference spectroscopy of the histones H1 and their trypsin-resistant cores from calf thymus and from the fruit fly Ceratitis capitata.
1980,
Pubmed
Bates,
Stability of the higher-order structure of chicken-erythrocyte chromatin in solution.
1981,
Pubmed
Boublík,
Optical rotatory dispersion and nuclear magnetic resonance. Studies of helix coil transitions in poly-L-arginine, poly-L-lysine and histones.
1970,
Pubmed
Bradbury,
Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. The conformation of histone H1.
1975,
Pubmed
Briand,
Chicken erythrocyte histone H5. IV. Sequence of the carboxy-termined half of the molecule (96 residues) and complete sequence.
1980,
Pubmed
Buehner,
Three-dimensional structure of D-glyceraldehyde-3-phosphate dehydrogenase.
1974,
Pubmed
Butler,
A defined structure of the 30 nm chromatin fibre which accommodates different nucleosomal repeat lengths.
1984,
Pubmed
Carroll,
Complexes of polylysine with polyuridylic acid and other polynucleotides.
1972,
Pubmed
Chou,
Prediction of protein conformation.
1974,
Pubmed
Clark,
Salt-dependent co-operative interaction of histone H1 with linear DNA.
1986,
Pubmed
Clore,
The polypeptide fold of the globular domain of histone H5 in solution. A study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics.
1987,
Pubmed
Coles,
Characterization of the chicken histone H1 gene complement. Generation of a complete set of vertebrate H1 protein sequences.
1987,
Pubmed
Compton,
Biochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes.
1976,
Pubmed
Crane-Robinson,
Structural studies of chicken erythrocyte histone H5.
1976,
Pubmed
De Petrocellis,
Structuring of H1 histone. Evidence of high-affinity binding sites for phosphate ions.
1986,
Pubmed
,
Echinobase
Dupressoir,
Isolation and characterization of five subfractions of chicken erythrocyte histone H1.
1984,
Pubmed
Garel,
Comparison between histones FV and F2a2 of chicken erythrocyte. I. Structure, stability and conformation of the free proteins.
1975,
Pubmed
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Giancotti,
Secondary and tertiary structural differences between histone H1 molecules from calf thymus and sea-urchin (Sphaerechinus granularis) sperm.
1981,
Pubmed
,
Echinobase
Greenfield,
Computed circular dichroism spectra for the evaluation of protein conformation.
1969,
Pubmed
Greenfield,
The use of computed optical rotatory dispersion curves for the evaluation of protein conformation.
1967,
Pubmed
Johns,
Studies on histones. 7. Preparative methods for histone fractions from calf thymus.
1964,
Pubmed
Liao,
Differences among subfractions of H1 histone in their interactions with linear and superhelical DNA. Circular dichroism.
1981,
Pubmed
Mirzabekov,
Protein arrangement in the DNA grooves in chromatin and nucleoprotamine in vitro and in vivo revealed by methylation.
1977,
Pubmed
Morris,
A comparison of the structure of chicken erythrocyte and chicken liver chromatin.
1976,
Pubmed
Morán,
Condensation of DNA by the C-terminal domain of histone H1. A circular dichroism study.
1985,
Pubmed
NEELIN,
THE HISTONES OF CHICKEN ERYTHROCYTE NUCLEI.
1964,
Pubmed
Poccia,
Transitions in histone variants during sea urchin spermatogenesis.
1987,
Pubmed
,
Echinobase
Provencher,
Estimation of globular protein secondary structure from circular dichroism.
1981,
Pubmed
Rifkind,
Helix--coil transition of poly-L-arginine: a comparison with other basic polypeptides.
1969,
Pubmed
Shannon,
Characterization of the six chicken histone H1 proteins and alignment with their respective genes.
1987,
Pubmed
Shimidzu,
Distinction and similarity in the structure of histones H1 and H5 as indicated by 13C nuclear-magnetic-resonance spectroscopy.
1985,
Pubmed
Strickland,
The primary structure of histone H1 from sperm of the sea urchin Parechinus angulosus. 2. Sequence of the C-terminal CNBr peptide and the entire primary structure.
1980,
Pubmed
,
Echinobase
Sugarman,
Genomic organization, DNA sequence, and expression of chicken embryonic histone genes.
1983,
Pubmed
,
Echinobase
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Thomas,
The study of histone--histone associations by chemical cross-linking.
1978,
Pubmed
Thomas,
Salt-induced folding of sea urchin sperm chromatin.
1986,
Pubmed
,
Echinobase
Tinoco,
The optical activity of nucleic acids and their aggregates.
1980,
Pubmed
Wagner,
Phosphorylation and dephosphorylation of histone (V (H5): controlled condensation of avian erythrocyte chromatin. Appendix: Phosphorylation and dephosphorylation of histone H5. II. Circular dichroic studies.
1977,
Pubmed
Warrant,
alpha-Helix-double helix interaction shown in the structure of a protamine-transfer RNA complex and a nucleoprotamine model.
1978,
Pubmed
Widom,
Higher-order structure of long repeat chromatin.
1985,
Pubmed
,
Echinobase
van Helden,
A theoretical consideration of the lysine-rich histones: H1 from a mammal and an echinoderm, H5 from erythrocytes.
1982,
Pubmed
,
Echinobase
