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Nucleic Acids Res
1990 Dec 25;1824:7339-48. doi: 10.1093/nar/18.24.7339.
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Positive and negative transcriptional regulatory elements in the early H4 histone gene of the sea urchin, Strongylocentrotus purpuratus.
Tung L
,
Lee IJ
,
Rice HL
,
Weinberg ES
.
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The early H4 (EH4) histone gene of the sea urchin, Strongylocentrotus purpuratus, is shown to contain at least five positive-responding sequence elements and one negative-responding site which control the level of in vitro transcription in an embryonic nuclear extract. The positive acting elements are: 1) the UHF-1 region, located between -133 and -102 (the site of a strong footprint, due at least in part to the binding of an 85 kD protein factor termed UHF-1); 2) the H4 specific element (H4SE), situated between -62 and -39; 3) a sequence corresponding to a TATA box between -33 and -26 (TAACAATA); 4) the transcriptional initiation site; and 5) an internal sequence element found between +19 and +50. Deletion of, or base changes in, the UHF-1, H4SE, initiation, or internal sequence sites resulted in significant decreases in transcription. Base substitutions in the TATA-like sequence had much less effect, resulting in no more than a 2-fold decrease in transcription, and there was no evidence that alternative initiation sites are utilized in the mutant templates. The negative element (termed the UHF-3 site) is contained within a footprinted region between nucleotides -75 and -56. Base substitutions in this area result in templates that were transcribed at a level 1.2-2.0-fold higher than the wild-type gene. Transcription levels of double UHF-1/H4SE and UHF-1/INR mutants were those expected from additive effects of the individual mutations and there was no suggestion of synergism.
Biggin,
Transcription factors that activate the Ultrabithorax promoter in developmentally staged extracts.
1988, Pubmed
Biggin,
Transcription factors that activate the Ultrabithorax promoter in developmentally staged extracts.
1988,
Pubmed
Busslinger,
Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.
1980,
Pubmed
,
Echinobase
Busslinger,
Synthesis of sperm and late histone cDNAs of the sea urchin with a primer complementary to the conserved 3' terminal palindrome: evidence for tissue-specific and more general histone gene variants.
1985,
Pubmed
,
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Carcamo,
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1990,
Pubmed
Chen,
Yeast mRNA initiation sites are determined primarily by specific sequences, not by the distance from the TATA element.
1985,
Pubmed
Childs,
Histone gene expression during sea urchin embryogenesis: isolation and characterization of early and late messenger RNAs of Strongylocentrotus purpuratus by gene-specific hybridization and template activity.
1979,
Pubmed
,
Echinobase
Childs,
Sea urchin (lytechinus pictus) late-stage histone H3 and H4 genes: characterization and mapping of a clustered but nontandemly linked multigene family.
1982,
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,
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Clerc,
Transcription of a cloned Xenopus laevis H4 histone gene in the homologous frog oocyte system depends on an evolutionary conserved sequence motif in the -50 region.
1983,
Pubmed
Concino,
The adenovirus major late promoter TATA box and initiation site are both necessary for transcription in vitro.
1984,
Pubmed
Dailey,
Distinct transcription factors bind specifically to two regions of the human histone H4 promoter.
1986,
Pubmed
Dailey,
Purification of the human histone H4 gene-specific transcription factors H4TF-1 and H4TF-2.
1988,
Pubmed
Grosschedl,
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
1980,
Pubmed
,
Echinobase
Grunstein,
Comparison of the early histone H4 gene sequence of Strongylocentrotus purpuratus with maternal, early, and late histone H4 mRNA sequences.
1981,
Pubmed
,
Echinobase
Grunstein,
Hatching in the sea urchin Lytechinus pictus is accompanied by a shift in histone H4 gene activity.
1978,
Pubmed
,
Echinobase
Hanly,
Identification of promoter elements necessary for transcriptional regulation of a human histone H4 gene in vitro.
1985,
Pubmed
Hariharan,
Equipotent mouse ribosomal protein promoters have a similar architecture that includes internal sequence elements.
1989,
Pubmed
Hariharan,
Functional dissection of a mouse ribosomal protein promoter: significance of the polypyrimidine initiator and an element in the TATA-box region.
1990,
Pubmed
Hentschel,
Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.
1980,
Pubmed
,
Echinobase
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Hieter,
Histone gene switch in the sea urchin embryo. Identification of late embryonic histone messenger ribonucleic acids and the control of their synthesis.
1979,
Pubmed
,
Echinobase
Hultmark,
Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22.
1986,
Pubmed
Hurt,
A region in the coding sequence is required for high-level expression of murine histone H3 gene.
1989,
Pubmed
Jones,
Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.
1988,
Pubmed
Kaumeyer,
Sequence, organization and expression of late embryonic H3 and H4 histone genes from the sea urchin, Strongylocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Kemler,
Characterization of two nonallelic pairs of late histone H2A and H2B genes of the sea urchin: differential regulation in the embryo and tissue-specific expression in the adult.
1986,
Pubmed
,
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Knowles,
Comparison of the late H1 histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Knowles,
Isolation, characterization, and expression of the gene encoding the late histone subtype H1-gamma of the sea urchin Strongylocentrotus purpuratus.
1987,
Pubmed
,
Echinobase
Knowles,
Temporal expression of late histone messenger RNA in the sea urchin Lytechinus pictus.
1984,
Pubmed
,
Echinobase
Kroeger,
Proximal and distal regulatory elements that influence in vivo expression of a cell cycle-dependent human H4 histone gene.
1987,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Lai,
Both basal and ontogenic promoter elements affect the timing and level of expression of a sea urchin H1 gene during early embryogenesis.
1988,
Pubmed
,
Echinobase
Lai,
Characterization of the structure and transcriptional patterns of the gene encoding the late histone subtype H1-beta of the sea urchin Strongylocentrotus purpuratus.
1988,
Pubmed
,
Echinobase
Mauron,
Accumulation of individual histone mRNAs during embryogenesis of the sea urchin Strongylocentrotus purpuratus.
1982,
Pubmed
,
Echinobase
Maxson,
Expression and organization of histone genes.
1983,
Pubmed
,
Echinobase
Maxson,
Evolution of late H2A, H2B, and H4 histone genes of the sea urchin, Strongylocentrotus purpuratus.
1987,
Pubmed
,
Echinobase
Maxson,
Accumulation of the early histone messenger RNAs during the development of Strongylocentrotus purpuratus.
1982,
Pubmed
,
Echinobase
Maxson,
Distinct organizations and patterns of expression of early and late histone gene sets in the sea urchin.
1983,
Pubmed
,
Echinobase
Morris,
A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III.
1983,
Pubmed
,
Echinobase
Morris,
Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos.
1986,
Pubmed
,
Echinobase
Mous,
Stimulation of sea urchin H2B histone gene transcription by a chromatin-associated protein fraction depends on gene sequences downstream of the transcription start site.
1985,
Pubmed
,
Echinobase
Nakatani,
An RNA polymerase II promoter containing sequences upstream and downstream from the RNA startpoint that direct initiation of transcription from the same site.
1990,
Pubmed
Overton,
Length and sequence heterogeneity of the histone gene repeat unit of the sea urchin, S. purpuratus.
1978,
Pubmed
,
Echinobase
Perkins,
In vitro analysis of the Antennapedia P2 promoter: identification of a new Drosophila transcription factor.
1988,
Pubmed
Perry,
Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements.
1985,
Pubmed
Roberts,
Sequence comparisons of non-allelic late histone genes and their early stage counterparts. Evidence for gene conversion within the sea urchin late stage gene family.
1984,
Pubmed
,
Echinobase
Schaffner,
Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
1978,
Pubmed
,
Echinobase
Sierra,
Structure and in vitro transcription of a human H4 histone gene.
1983,
Pubmed
Smale,
The "initiator" as a transcription control element.
1989,
Pubmed
Smale,
Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
1990,
Pubmed
Soeller,
In vitro transcription of the Drosophila engrailed gene.
1988,
Pubmed
Sugarman,
Genomic organization, DNA sequence, and expression of chicken embryonic histone genes.
1983,
Pubmed
,
Echinobase
Sures,
Leader sequences of Strongylocentrotus purpuratus histone mRNAs start at a unique heptanucleotide common to all five histone genes.
1980,
Pubmed
,
Echinobase
Talkington,
Rescuing the in vitro function of a globin pseudogene promoter.
1982,
Pubmed
Tebb,
Positionally exact initiation is required for the formation of a stable RNA polymerase II transcription complex in vivo.
1988,
Pubmed
Tokunaga,
In monkey COS cells only the TATA box and the cap site region are required for faithful and efficient initiation of the fibroin gene transcription.
1984,
Pubmed
Tung,
Sea urchin early and late H4 histone genes bind a specific transcription factor in a stable preinitiation complex.
1989,
Pubmed
,
Echinobase
Uzman,
The role of RNA polymerase initiation and elongation in control of total RNA and histone mRNA synthesis in sea urchin embryos.
1984,
Pubmed
,
Echinobase
Vieira,
Production of single-stranded plasmid DNA.
1987,
Pubmed
Wang,
Inverted duplication of histone genes in chicken and disposition of regulatory sequences.
1985,
Pubmed
Weinberg,
Timing and rates of synthesis of early histone mRNA in the embryo of Strongylocentrotus purpuratus.
1983,
Pubmed
,
Echinobase
Wells,
Compilation analysis of histones and histone genes.
1986,
Pubmed
Winkfein,
Histone H4 and H2B genes in rainbow trout (Salmo gairdnerii).
1985,
Pubmed
Yager,
Evolving sea urchin histone genes--nucleotide polymorphisms in the H4 gene and spacers of Strongylocentrotus purpuratus.
1984,
Pubmed
,
Echinobase
Zhong,
The primary structure and expression of four cloned human histone genes.
1983,
Pubmed
van Wijnen,
Human H4 histone gene transcription requires the proliferation-specific nuclear factor HiNF-D. Auxiliary roles for HiNF-C (Sp1-like) and HiNF-A (high mobility group-like).
1989,
Pubmed
van Wijnen,
A nuclear protein with affinity for the 5' flanking region of a cell cycle dependent human H4 histone gene in vitro.
1987,
Pubmed
