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Cell Regul
1990 May 01;16:465-9. doi: 10.1091/mbc.1.6.465.
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Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor.
Shilling F
,
Mandel G
,
Jaffe LA
.
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Starfish oocytes were injected with mRNA for the serotonin 1c receptor or with rat brain poly A+ mRNA, incubated to allow expression of the membrane protein, then matured to eggs by addition of 1-methyladenine. Applying serotonin to these eggs caused cortical granule exocytosis like that occurring at fertilization. Because the serotonin 1c receptor specifically activates a G-protein, these results provide support for the hypothesis that sperm activate eggs by way of a receptor-G-protein interaction. The starfish oocyte may be a generally useful system for expression of exogenous mRNA for membrane proteins.
Bonner,
Identification of a family of muscarinic acetylcholine receptor genes.
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Bonner,
Identification of a family of muscarinic acetylcholine receptor genes.
1987,
Pubmed
Colin,
Injected mRNA does not increase protein synthesis in unfertilized, fertilized, or ammonia-activated sea urchin eggs.
1986,
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,
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Cooperman,
Modulation of sodium-channel mRNA levels in rat skeletal muscle.
1987,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
Hagiwara,
Electrical properties of egg cell membranes.
1979,
Pubmed
Hagiwara,
Voltage clamp analysis of two inward current mechanisms in the egg cell membrane of a starfish.
1975,
Pubmed
,
Echinobase
Hiramoto,
A method of microinjection.
1974,
Pubmed
Jones,
Electrophysiological characterization of cloned m1 muscarinic receptors expressed in A9 L cells.
1988,
Pubmed
Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
Kiehart,
Microinjection of echinoderm eggs: apparatus and procedures.
1982,
Pubmed
,
Echinobase
Kishimoto,
Microinjection and cytoplasmic transfer in starfish oocytes.
1986,
Pubmed
,
Echinobase
Kline,
Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs.
1988,
Pubmed
Konarska,
Recognition of cap structure in splicing in vitro of mRNA precursors.
1984,
Pubmed
Litosch,
5-Methyltryptamine stimulates phospholipase C-mediated breakdown of exogenous phosphoinositides by blowfly salivary gland membranes.
1985,
Pubmed
McCloskey,
Cholera toxin potentiates IgE-coupled inositol phospholipid hydrolysis and mediator secretion by RBL-2H3 cells.
1988,
Pubmed
Moody,
The development of calcium and potassium currents during oogenesis in the starfish, Leptasterias hexactis.
1985,
Pubmed
,
Echinobase
Saito,
Glutamate receptors expressed in Xenopus oocyte by messenger RNA from invertebrate muscle.
1987,
Pubmed
Shapiro,
Isolation, sequence, and functional expression of the mouse M1 muscarinic acetylcholine receptor gene.
1988,
Pubmed
Turner,
A cholera toxin-sensitive G-protein stimulates exocytosis in sea urchin eggs.
1987,
Pubmed
,
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Turner,
Regulation of cortical vesicle exocytosis in sea urchin eggs by inositol 1,4,5-trisphosphate and GTP-binding protein.
1986,
Pubmed
,
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