Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1991 Oct 01;1151:67-73. doi: 10.1083/jcb.115.1.67.
Show Gene links
Show Anatomy links
Dual-view microscopy with a single camera: real-time imaging of molecular orientations and calcium.
Kinosita K
,
Itoh H
,
Ishiwata S
,
Hirano K
,
Nishizaka T
,
Hayakawa T
.
???displayArticle.abstract???
A new microscope technique, termed "W" (double view video) microscopy, enables simultaneous observation of two different images of an object through a single video camera or by eye. The image pair may, for example, be transmission and fluorescence, fluorescence at different wavelengths, or mutually perpendicular components of polarized fluorescence. Any video microscope can be converted into a dual imager by simple insertion of a small optical device. The continuous appearance of the dual image assures the best time resolution in existing and future video microscopes. As an application, orientations of actin protomers in individual, moving actin filaments have been imaged at the video rate. Asymmetric calcium influxes into a cell exposed to an intense electric pulse have also been visualized.
Axelrod,
Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization.
1979, Pubmed
Axelrod,
Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization.
1979,
Pubmed
Foskett,
Simultaneous Nomarski and fluorescence imaging during video microscopy of cells.
1988,
Pubmed
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Harada,
Sliding movement of single actin filaments on one-headed myosin filaments.
1987,
Pubmed
Hibino,
Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential.
1991,
Pubmed
,
Echinobase
Kinosita,
Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.
1988,
Pubmed
,
Echinobase
Kinosita,
A theory of fluorescence polarization decay in membranes.
1977,
Pubmed
Masai,
Dynamic light-scattering study on polymerization process of muscle actin.
1986,
Pubmed
Prochniewicz-Nakayama,
Studies on conformation of F-actin in muscle fibers in the relaxed state, rigor, and during contraction using fluorescent phalloidin.
1983,
Pubmed
Rossignol,
Induction of calcium-dependent, localized cortical granule breakdown in sea-urchin eggs by voltage pulsation.
1983,
Pubmed
,
Echinobase
Sowers,
Fusion events and nonfusion contents mixing events induced in erythrocyte ghosts by an electric pulse.
1988,
Pubmed
Toyoshima,
Myosin subfragment-1 is sufficient to move actin filaments in vitro.
1987,
Pubmed
Tsien,
Fluorescence measurement and photochemical manipulation of cytosolic free calcium.
1988,
Pubmed
Tsong,
Voltage modulation of membrane permeability and energy utilization in cells.
1983,
Pubmed
Weeds,
Studies on the chymotryptic digestion of myosin. Effects of divalent cations on proteolytic susceptibility.
1977,
Pubmed
Zimmermann,
Electric field-mediated fusion and related electrical phenomena.
1982,
Pubmed
