ECB-ART-52369Dev Growth Differ 1982 Jan 01;242:163-171. doi: 10.1111/j.1440-169X.1982.00163.x.
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Acid Release during Activation of Barnea candida (Mollusca, Pelecypoda) Oocytes: (acid release/oocyte activation/Barnea candida).
Barnea caridida oocytes release acid (1.35 pmole H+ /oocyte) upon fertilization. After artificial activation by an excess of KCl, germinal vesicle breakdown (GVBD) occurs normally and a quite similar, but not identical, acid release is recorded (1.10 pmole H+ /oocyte). KCl activation of Barnea oocytes is completely inhibited in 100 mM sodium-acetate sea water at pH 6.5 and fertilization does not result in activation when the oocytes are transferred after one minute into 100 mM sodium-acetate sea water at pH 6.3. When D-600, a calcium transmembrane fluxes inhibitor, is added 20 seconds after fertilization, GVBD is inhibited but a normal acid release is recorded. The presence of at least 10 mM sodium ions in the external medium is required for 100% activation of these oocytes by an excess of KCl. These results suggest that while an intracellular pH increase may be a requisite for GVBD, this can not be a sufficient condition to trigger it unless a calcium influx is allowed to occur. Moreover, the acid release does not result from a Ca++ -H+ exchange transport but appears more likely to be due to a Na+ -H* exchange as it has been demontrated in sea urchin eggs.
PubMed ID: 37282037
Article link: Dev Growth Differ