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Biology (Basel)
2021 Dec 30;111:. doi: 10.3390/biology11010050.
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Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus).
Cui W
,
Huo D
,
Liu S
,
Xing L
,
Su F
,
Yang H
,
Sun L
.
Abstract
Genetic linkage maps have become an indispensable tool for genetics and genomics research. Sea cucumber (Apostichopus japonicus), which is an economically important mariculture species in Asia, is an edible echinoderm with medicinal properties. In this study, the first SNP-based high-density genetic linkage map was constructed by sequencing 132 A. japonicus individuals (2 parents and 130 offspring) according to a genotyping-by-sequencing (GBS) method. The consensus map was 3181.54 cM long, with an average genetic distance of 0.52 cM. A total of 6144 SNPs were assigned to 22 linkage groups (LGs). A Pearson analysis and QTL mapping revealed the correlations among body weight, body length, and papillae number. An important growth-related candidate gene, protein still life, isoforms C/SIF type 2 (sif), was identified in LG18. The gene was significantly highly expressed during the larval developmental stages. Its encoded protein reportedly functions as a guanine nucleotide exchange factor. These results would facilitate the genetic analysis of growth traits and provide valuable genomic resources for the selection and breeding of new varieties of sea cucumbers with excellent production traits.
2018YFD0900105 National Key R&D Program of China, 42076093，42030408 National Natural Science Foundation of China, XDA24030304 Strategic Priority Research Program of the Chinese Academy of Sciences, 2019209 Youth Innovation Promotion Association CAS
Figure 1. Consensus linkage map of Apostichopus japonicus. Blue bars represent SNPs. The scale on the left indicates the genetic distance in centimorgans (cM).
Figure 2. The frequency distribution of phenotypic data. (a) Body length; (b) body weight; (c) papillae number.
Figure 3. Correlations among three growth traits in Apostichopus japonicus.
Figure 4. (a) Expression characteristics of the sif gene in different Apostichopus japonicus larval developmental stages. (b) Expression characteristics of the sif gene in different adult Apostichopus japonicus tissues. Asterisks indicate significant differences between the groups (* p < 0.05, ** p < 0.01).
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