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ECB-ART-46460
Carbohydr Res 2018 Aug 01;466:11-17. doi: 10.1016/j.carres.2018.07.001.
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Development and application of a HPLC-MS/MS method for quantitation of fucosylated chondroitin sulfate and fucoidan in sea cucumbers.

Zhu Z , Zhu B , Ai C , Lu J , Wu S , Liu Y , Wang L , Yang J , Song S , Liu X .


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Fucosylated chondroitin sulfate (FCS) and fucoidan (FUC) are two main bioactive polysaccharides in sea cucumbers. A novel method for quantitation of FCS and FUC was developed by detecting chondroitin disaccharide and fucose produced through acid hydrolysis using HPLC-MS/MS. The present method showed satisfactory performance for both saccharides. It was applied to assay sea cucumbers (Stichopus japonicus) reared in pond grow-out or bottom sowing, and the results were compared with those obtained by traditional HPLC method and 1,9-dimethylmethylene blue test, which could only provide the total sea cucumber polysaccharide (SCP) contents. No difference of total SCP content was observed between sea cucumbers reared through different ways, while a higher ratio of FCS to FUC of sea cucumbers of pond grow-out was revealed by the present method. Thus, this novel method is potential to quantify the two polysaccharides and could be a powerful tool for quality evaluation of sea cucumbers.

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Genes referenced: LOC100887844