J Cell Sci 1978 Feb 01;29:171-88. doi: 10.1242/jcs.29.1.171.

Immunofluorescent anti-tubulin staining of spindles during meiotic maturation of mouse oocytes in vitro.

???displayArticle.abstract???
Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing mouse oocytes. Changes in the tubulin-specific staining pattern during meiotic maturation in vitro take place in a highly reproducible manner. Oocytes exposed continuously to cytochalasin B arrest at metaphase I and display a spindle which by immunofluorescent staining is virtually indistinguishable from the spindle of untreated oocytes.

???displayArticle.pubmedLink??? 75210
???displayArticle.link??? J Cell Sci


Genes referenced: LOC100887844 tubgcp2