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J Proteome Res
2010 Feb 05;92:990-6. doi: 10.1021/pr900885k.
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A hybrid, de novo based, genome-wide database search approach applied to the sea urchin neuropeptidome.
Menschaert G
,
Vandekerckhove TT
,
Baggerman G
,
Landuyt B
,
Sweedler JV
,
Schoofs L
,
Luyten W
,
Van Criekinge W
.
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Peptidomics is the identification and study of the in vivo biologically active peptide profile. A combination of high performance liquid chromatography, mass spectrometry, and bioinformatics tools such as database search engines are commonly used to perform the analysis. We report a methodology based on a database system holding the completed translated genome, whereby de novo sequencing and genome-wide database searching are combined. The methodology was applied to the sea urchin neuropeptidome resulting in a 30% increase in identification rate.
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20000637
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