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The genomic cis-regulatory systems controlling regulatory gene expression usually include multiple modules. The regulatory output of such systems at any given time depends on which module is directing the function of the basal transcription apparatus, and ultimately on the transcription factor inputs into that module. Here we examine regulation of the Strongylocentrotus purpuratus tbrain gene, a required activator of the skeletogenic specification state in the lineage descendant from the embryo micromeres. Alternate cis-regulatory modules were found to convey skeletogenic expression in reporter constructs. To determine their relative developmental functions in context, we made use of recombineered BAC constructs containing a GFP reporter and of derivatives from which specific modules had been deleted. The outputs of the various constructs were observed spatially by GFP fluorescence and quantitatively over time by QPCR. In the context of the complete genomic locus, early skeletogenic expression is controlled by an intron enhancer plus a proximal region containing a HesC site as predicted from network analysis. From ingression onward, however, a dedicated distal module utilizing positive Ets1/2 inputs contributes to definitive expression in the skeletogenic mesenchyme. This module also mediates a newly discovered negative Erg input which excludes non-skeletogenic mesodermal expression.
Cameron,
cis-Regulatory activity of randomly chosen genomic fragments from the sea urchin.
2004, Pubmed,
Echinobase
Cameron,
cis-Regulatory activity of randomly chosen genomic fragments from the sea urchin.
2004,
Pubmed
,
Echinobase
Consales,
Functional characterization of Ets-binding sites in the sea urchin embryo: three base pair conversions redirect expression from mesoderm to ectoderm and endoderm.
2002,
Pubmed
,
Echinobase
Croce,
ske-T, a T-box gene expressed in the skeletogenic mesenchyme lineage of the sea urchin embryo.
2001,
Pubmed
,
Echinobase
Damle,
Confocal quantification of cis-regulatory reporter gene expression in living sea urchin.
2006,
Pubmed
,
Echinobase
Flytzanis,
Persistence and integration of cloned DNA in postembryonic sea urchins.
1985,
Pubmed
,
Echinobase
Fuchikami,
T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo.
2002,
Pubmed
,
Echinobase
Gao,
Transfer of a large gene regulatory apparatus to a new developmental address in echinoid evolution.
2008,
Pubmed
,
Echinobase
Gray,
Short-range repression permits multiple enhancers to function autonomously within a complex promoter.
1994,
Pubmed
Hinman,
Evolutionary plasticity of developmental gene regulatory network architecture.
2007,
Pubmed
,
Echinobase
Hinman,
Caught in the evolutionary act: precise cis-regulatory basis of difference in the organization of gene networks of sea stars and sea urchins.
2007,
Pubmed
,
Echinobase
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Iso,
HES and HERP families: multiple effectors of the Notch signaling pathway.
2003,
Pubmed
Kurokawa,
HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation in the sea urchin embryo.
1999,
Pubmed
,
Echinobase
Lee,
Exclusive developmental functions of gatae cis-regulatory modules in the Strongylocentrorus purpuratus embryo.
2007,
Pubmed
,
Echinobase
Longabaugh,
Visualization, documentation, analysis, and communication of large-scale gene regulatory networks.
2009,
Pubmed
Mao,
Multiple Otx binding sites required for expression of the Strongylocentrotus purpuratus Spec2a gene.
1994,
Pubmed
,
Echinobase
Maruyama,
A Sea Cucumber Homolog of the Mouse T-Brain-1 is Expressed in the Invaginated Cells of the Early Gastrula in Holothuria leucospilota.
2000,
Pubmed
,
Echinobase
Materna,
A protocol for unraveling gene regulatory networks.
2008,
Pubmed
,
Echinobase
McMahon,
Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis.
1985,
Pubmed
,
Echinobase
Ochiai,
Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus.
2008,
Pubmed
,
Echinobase
Oliveri,
A regulatory gene network that directs micromere specification in the sea urchin embryo.
2002,
Pubmed
,
Echinobase
Oliveri,
Global regulatory logic for specification of an embryonic cell lineage.
2008,
Pubmed
,
Echinobase
Oliveri,
Activation of pmar1 controls specification of micromeres in the sea urchin embryo.
2003,
Pubmed
,
Echinobase
Ransick,
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
2002,
Pubmed
,
Echinobase
Revilla-i-Domingo,
A missing link in the sea urchin embryo gene regulatory network: hesC and the double-negative specification of micromeres.
2007,
Pubmed
,
Echinobase
Revilla-i-Domingo,
R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres.
2004,
Pubmed
,
Echinobase
Rizzo,
Identification and developmental expression of the ets gene family in the sea urchin (Strongylocentrotus purpuratus).
2006,
Pubmed
,
Echinobase
Shoguchi,
A starfish homolog of mouse T-brain-1 is expressed in the archenteron of Asterina pectinifera embryos: possible involvement of two T-box genes in starfish gastrulation.
2000,
Pubmed
,
Echinobase
Smith,
Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo.
2008,
Pubmed
,
Echinobase
Smith,
A new method, using cis-regulatory control, for blocking embryonic gene expression.
2008,
Pubmed
,
Echinobase
Wang,
Maternal and embryonic provenance of a sea urchin embryo transcription factor, SpZ12-1.
1995,
Pubmed
,
Echinobase
Warming,
Simple and highly efficient BAC recombineering using galK selection.
2005,
Pubmed
Yon,
Precise gene fusion by PCR.
1989,
Pubmed
Yuh,
Modular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo.
1996,
Pubmed
,
Echinobase
Yuh,
Complexity and organization of DNA-protein interactions in the 5'-regulatory region of an endoderm-specific marker gene in the sea urchin embryo.
1994,
Pubmed
,
Echinobase
Yuh,
Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene.
1996,
Pubmed
,
Echinobase
