ECB-ART-29959
Biochim Biophys Acta
1976 Apr 23;4282:525-31. doi: 10.1016/0304-4165(76)90061-1.
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Subcellular localizations of guanylate cyclase and 3'',5''-cyclic nucleotide phosphodiesterase in sea urchin sperm.
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The subcellular localizations of guanylate cyclase and 3'',5''-cyclic nucleotide phosphodiesterase in sea urchin sperm were examined. Both the specific and total activities of these two enzymes were much higher in sperm flagella (tails) than in the heads. In addition to the observation that guanylate cyclase in the flagella was particulate-bound and solubilized by Triton X-100, more than 80% of the cyclase activity in the flagella was found in the plasma membrane fraction, whereas the activity of cyclic nucleotide phosphodiesterase was observed in both the axonemal and plasma membrane fractions. The observations indicated that the cyclase in the flagella appeared to be associated with the plasma membrane. Cyclic nucleotide phosphodiesterase in the plasma membrane fraction as well as the axonemal fraction hydrolyzed both cyclic GMP and cyclic AMP; however, the rates of hydrolysis for cyclic GMP were obviously higher than those for cyclic AMP. The enzymic properties of guanylate cyclase and cyclic nucleotide phosphodiesterase in sperm flagella were also briefly described.
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Genes referenced: dnah3 LOC100887844 LOC576733