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Membrane fusion events are required in three steps in sea urchin fertilization: the acrosome reaction in sperm, fusion of the plasma membrane of acrosome-reacted sperm with the plasma membrane of the egg, and exocytosis of the contents of the egg cortical granules. We recently reported the involvement of a Zn2+-dependent metalloendoprotease in the acrosome reaction (Farach, H. C., D. I. Mundy, W. J. Strittmatter, and W. J. Lennarz. 1987. J. Biol. Chem. 262:5483-5487). In the current study, we investigated the possible involvement of metalloendoproteases in the two other fusion events of fertilization. The use of inhibitors of metalloendoproteases provided evidence that at least one of the fusion events subsequent to the acrosome reaction requires such enzymes. These inhibitors did not block the binding of sperm to egg or the process of cortical granule exocytosis. However, sperm-egg fusion, assayed by the ability of the bound sperm to establish cytoplasmic continuity with the egg, was inhibited by metalloendoprotease substrate. Thus, in addition to the acrosome reaction, an event in the gamete fusion process requires a metalloendoprotease.
Clapper,
Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm.
1985, Pubmed,
Echinobase
Clapper,
Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm.
1985,
Pubmed
,
Echinobase
Couch,
Rat myoblast fusion requires metalloendoprotease activity.
1983,
Pubmed
Couch,
Specific blockers of myoblast fusion inhibit a soluble and not the membrane-associated metalloendoprotease in myoblasts.
1984,
Pubmed
Decker,
Sperm binding and fertilization envelope formation in a cell surface complex isolated from sea urchin eggs.
1979,
Pubmed
,
Echinobase
Fabiato,
Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells.
1979,
Pubmed
Farach,
Evidence for the involvement of metalloendoproteases in the acrosome reaction in sea urchin sperm.
1987,
Pubmed
,
Echinobase
Green,
Ultrastructural demonstration of trypsin-like protease in acrosomes of sea urchin sperm.
1980,
Pubmed
,
Echinobase
Hinkley,
Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342.
1986,
Pubmed
,
Echinobase
Jackson,
Mild proteolytic digestion restores exocytotic activity to N-ethylmaleimide-inactivated cell surface complex from sea urchin eggs.
1985,
Pubmed
,
Echinobase
Kinsey,
Studies on the specificity of sperm binding in echinoderm fertilization.
1980,
Pubmed
,
Echinobase
Kinsey,
The effect of the acrosome reaction on the respiratory activity and fertilizing capacity of echinoid sperm.
1979,
Pubmed
,
Echinobase
Mumford,
A zinc metalloendopeptidase associated with dog pancreatic membranes.
1980,
Pubmed
Mundy,
Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.
1985,
Pubmed
Resing,
A 53,000-Da esterase in Strongylocentrotus purpuratus semen is derived from phagocytic cells, not sperm.
1985,
Pubmed
,
Echinobase
Rossignol,
Characterization of the sperm receptor on the surface of eggs of Strongylocentrotus purpuratus.
1984,
Pubmed
,
Echinobase
SeGall,
Chemical characterization of the component of the jelly coat from sea urchin eggs responsible for induction of the acrosome reaction.
1979,
Pubmed
,
Echinobase
Trimmer,
Activation of sea urchin gametes.
1986,
Pubmed
,
Echinobase
White,
Membrane fusion proteins of enveloped animal viruses.
1983,
Pubmed
