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Molecules
2018 Mar 06;233:. doi: 10.3390/molecules23030590.
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Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.
Qin Y
,
Yuan Q
,
Zhang Y
,
Li J
,
Zhu X
,
Zhao L
,
Wen J
,
Liu J
,
Zhao L
,
Zhao J
.
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Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.
Figure 1. Effects of different extraction parameters such as enzyme concentration (A), temperature (B), ratio of solvent to raw material (C), pH (D), and time (E) on the yield of polysaccharides from sea cucumber Phyllophorus proteus. ★ indicates the optimal reaction condition for each single-factor test.
Figure 2. Response surface plots (A, C and E) and contour plots (B, D and F) showing the effects of variables (X1, ratio of extraction solvent to raw material; X2, extraction time; X3, extraction pH) and their mutual effects on the extraction yield of PPP.
Figure 3. HPLC profiles of PPP (A), PPP-1a (B), PPP-2 (C) and PPP-1b (D) (a), chromatograms of PMP derivatives of mixed monosaccharide standards (A), PPP-1a (B), PPP-1b (C), PPP-2 (D) and PPP (E) (b), and conductimetric titration curves of PPP-1a, PPP-1b and PPP-2 (c).
Figure 4. FI-IR (A) and 1H-NMR (B) spectra of PPP-1a, PPP-1b and PPP-2.
Figure 5. Scavenging effects of PPP, PPP-1a, PPP-1b and PPP-2 on DPPH radical (A), hydroxyl radical (B), superoxide radical (C) and ABTS radical (D), respectively.
Barnes,
Deep-sea macroplanktonic sea cucumbers: suspended sediment feeders captured from deep submergence vehicle.
1976, Pubmed,
Echinobase
Barnes,
Deep-sea macroplanktonic sea cucumbers: suspended sediment feeders captured from deep submergence vehicle.
1976,
Pubmed
,
Echinobase
Bi,
Structural elucidation and antioxidant activity of a water-soluble polysaccharide from the fruit bodies of Bulgaria inquinans (Fries).
2013,
Pubmed
Bordbar,
High-value components and bioactives from sea cucumbers for functional foods--a review.
2011,
Pubmed
,
Echinobase
Cao,
Structural characterization of immunostimulating protein-sulfated fucan complex extracted from the body wall of a sea cucumber, Stichopus japonicus.
2017,
Pubmed
,
Echinobase
Casu,
A conductimetric method for the determination of sulphate and carboxyl groups in heparin and other mucopolysaccharides.
1975,
Pubmed
Chen,
Sequence determination and anticoagulant and antithrombotic activities of a novel sulfated fucan isolated from the sea cucumber Isostichopus badionotus.
2012,
Pubmed
,
Echinobase
Chen,
Optimization of enzyme assisted extraction of polysaccharides from Astragalus membranaceus.
2014,
Pubmed
Duan,
Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids.
2016,
Pubmed
,
Echinobase
Esmat,
Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats.
2013,
Pubmed
,
Echinobase
Guo,
Ultrasound effects on the degradation kinetics, structure, and antioxidant activity of sea cucumber fucoidan.
2014,
Pubmed
,
Echinobase
He,
Sea cucumber (Codonopsis pilosula) oligopeptides: immunomodulatory effects based on stimulating Th cells, cytokine secretion and antibody production.
2016,
Pubmed
,
Echinobase
Jin,
Characterization and antioxidant activity of a polysaccharide extracted from Sarcandra glabra.
2012,
Pubmed
Li,
Extraction of Opuntia dillenii Haw. Polysaccharides and Their Antioxidant Activities.
2016,
Pubmed
Li,
Macromolecular properties and hypolipidemic effects of four sulfated polysaccharides from sea cucumbers.
2017,
Pubmed
,
Echinobase
Liu,
Purification and antioxidant activity of a polysaccharide from bulbs of Fritillaria ussuriensis Maxim.
2012,
Pubmed
Liu,
Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus.
2012,
Pubmed
,
Echinobase
Liu,
In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus).
2013,
Pubmed
Liu,
In Vivo Anti-Cancer Mechanism of Low-Molecular-Weight Fucosylated Chondroitin Sulfate (LFCS) from Sea Cucumber Cucumaria frondosa.
2016,
Pubmed
,
Echinobase
Liu,
Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue.
2016,
Pubmed
Luo,
Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers.
2013,
Pubmed
,
Echinobase
Mena-Bueno,
Sea cucumbers with an anti-inflammatory effect on endothelial cells and subcutaneous but not on epicardial adipose tissue.
2016,
Pubmed
,
Echinobase
Mulloy,
Sulfated fucans from echinoderms have a regular tetrasaccharide repeating unit defined by specific patterns of sulfation at the 0-2 and 0-4 positions.
1994,
Pubmed
,
Echinobase
Myron,
Fucosylated chondroitin sulfate diversity in sea cucumbers: a review.
2014,
Pubmed
,
Echinobase
Panagos,
Fucosylated chondroitin sulfates from the body wall of the sea cucumber Holothuria forskali: conformation, selectin binding, and biological activity.
2014,
Pubmed
,
Echinobase
Pomin,
Holothurian fucosylated chondroitin sulfate.
2014,
Pubmed
,
Echinobase
Re,
Antioxidant activity applying an improved ABTS radical cation decolorization assay.
1999,
Pubmed
Saito,
Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates.
1968,
Pubmed
Silchenko,
Structures and Biogenesis of Fallaxosides D₄, D₅, D₆ and D₇, Trisulfated Non-Holostane Triterpene Glycosides from the Sea Cucumber Cucumaria fallax.
2016,
Pubmed
,
Echinobase
Tako,
Chemical Characterization of Fucoidan from Commercially Cultured Nemacystus decipiens (Itomozuku).
1999,
Pubmed
Ustyuzhanina,
The structure of a fucosylated chondroitin sulfate from the sea cucumber Cucumaria frondosa.
2017,
Pubmed
,
Echinobase
Ustyuzhanina,
Structural characterization of fucosylated chondroitin sulfates from sea cucumbers Apostichopus japonicus and Actinopyga mauritiana.
2016,
Pubmed
,
Echinobase
Wang,
Fucoidan from sea cucumber Cucumaria frondosa exhibits anti-hyperglycemic effects in insulin resistant mice via activating the PI3K/PKB pathway and GLUT4.
2016,
Pubmed
,
Echinobase
Wei,
In vitro fermentation behaviors of fucosylated chondroitin sulfate from Pearsonothuria graeffei by human gut microflora.
2017,
Pubmed
,
Echinobase
Wu,
Depolymerization of fucosylated chondroitin sulfate from sea cucumber, Pearsonothuria graeffei, via 60Co irradiation.
2013,
Pubmed
,
Echinobase
Wu,
Differentiation of dried sea cucumber products from different geographical areas by surface desorption atmospheric pressure chemical ionization mass spectrometry.
2009,
Pubmed
,
Echinobase
Wu,
Structural analysis and anticoagulant activities of the novel sulfated fucan possessing a regular well-defined repeating unit from sea cucumber.
2015,
Pubmed
,
Echinobase
Wu,
Sulfation pattern of fucose branches affects the anti-hyperlipidemic activities of fucosylated chondroitin sulfate.
2016,
Pubmed
,
Echinobase
Yang,
Hyperbranched acidic polysaccharide from green tea.
2010,
Pubmed
You,
Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities.
2013,
Pubmed
Yu,
Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas.
2014,
Pubmed
,
Echinobase
Yuan,
Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves.
2015,
Pubmed
Zhang,
Ultrasound enhanced production and antioxidant activity of polysaccharides from mycelial fermentation of Phellinus igniarius.
2014,
Pubmed
Zhao,
Discovery of an intrinsic tenase complex inhibitor: Pure nonasaccharide from fucosylated glycosaminoglycan.
2015,
Pubmed
Zhao,
Digestibility of fucosylated glycosaminoglycan from sea cucumber and its effects on digestive enzymes under simulated salivary and gastrointestinal conditions.
2018,
Pubmed
,
Echinobase
Zhao,
In vitro antioxidant activity of extracts from common legumes.
2014,
Pubmed
Zhao,
Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage.
2013,
Pubmed
,
Echinobase
Zhu,
Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus.
2014,
Pubmed
Zhu,
Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel.
2015,
Pubmed
