ECB-ART-36571
Carbohydr Res
1997 Jan 17;2973:273-9. doi: 10.1016/s0008-6215(96)00258-3.
Show Gene links
Show Anatomy links
Structure of fucose branches in the glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus.
???displayArticle.abstract???
Fucose-branched chondroitin sulfate E was prepared from the body wall of sea cucumber Stichopus japonicus. The purified glycosaminoglycan (GAG) was chemically desulfated, followed by carboxyl reduction. Intact, desulfated, and desulfated/carboxyl-reduced GAG fractions were subjected to per-O-methylation. GC-MS analyses of the resultant partially methylated alditol acetates demonstrated that the fucose branch is formed by two fucopyranosyl residues linked glycosidically through position (1-->3), and that the fucose branch and glucuronic acid are almost equimolar. In addition, it was elucidated that about 20% of the branches stretch from O-3 position of a glucuronic acid moiety of the core chondroitin sulfate polymer, while remaining fucose branches are postulated to protrude from O-4 and/or O-6 position(s) of a N-acetylgalactosamine moiety. This fucose branch was also confirmed to be highly sulfated according to six kinds of substitution pattern in methylation analysis.
???displayArticle.pubmedLink??? 9060189
???displayArticle.link??? Carbohydr Res
Genes referenced: LOC100887844