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Echinobase
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Echinobase Accession
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Echinoderm mRNA
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S. purpuratus
(Purple sea urchin) 5.0
L. variegatus
(Green sea urchin) 3.0
P. miniata
(Bat star) 3.0
A. planci
(Crown-of-thorns) 1.0
L. pictus
(Painted urchin) 3.0
A. rubens
(Sugar star) 1.3
A. japonica
(Feather star) 1.0
Genomes
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S. purpuratus
(Purple sea urchin) 5.0
L. variegatus
(Green sea urchin) 3.0
P. miniata
(Bat star) 3.0
A. planci
(Crown-of-thorns) 1.0
L. pictus
(Painted urchin) 3.0
A. rubens
(Sugar star) 1.3
A. japonica
(Feather star) 1.0
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Download BLAST databases
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???blast.program.blastp???
???blast.program.blastx???
???blast.program.tblastx???
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-- Genomes --
Strongylocentrotus purpuratus 5.0 Genome
Strongylocentrotus purpuratus 3.1 Genome
Lytechinus variegatus 3.0 Genome
Patiria miniata 3.0 Genome
Acanthaster planci 1.0 Genome
Lytechinus pictus 3.0 Genome
Lytechinus pictus 2.1 Genome
Asterias rubens 1.3 Genome
Anneissia japonica 1.0 Genome
-- mRNA --
Echinoderm mRNA
-- CDS --
Echinoderm CDS
-- Protein --
Echinoderm Protein
???blast.query???
>JT099333.1 TSA: Strongylocentrotus purpuratus WHL22.354032.0 mRNA sequence ATTGTCCTGGCGGATATTACTGTGAGAGCCCAGGCCAAGATTCAACGACCGACGAATGCGACCCGGGATT CTTCTGTACTCTAGGTGTGAACGTACGAGCTCCTAATGGGCAGAATAATACCGGGGTCGGAGGTCATTGC TACGCAGGTCACGAGTGTCCGACGGGAACAGCTTCTCCTGTCCCATGTCTGGCTGGGTTCTACAGCGGCT ACACGCAGAGAGCTAGCTGTGATGTTTGCCCACCAGGCTATTACTGTGAGAATGCCACTGTTGTACCTCT GGAATGTCCAGTCGGTCACTACTGTCCAGACGGAACCGATTACAGTGACCAGTACCCTTGCCCAGCCGGA ACATATAACAATGTCACAGGTCAAATGAATCTGACCGACTGCATGTTCTGCCCACCTGGCATGTACTGTG AAGGAACAGGCTTAGACTACCCCAGCGGTTGGTGCGACGAAGGATTCTTCTGCACAGGAGGCTCCTATGC CAAGAGACCATTCAACGTAGGCGTTCTAGTCAACAACAGCTACCAGTATCCAGATGATACCTGTCACCCA CAGTTTGACTGCGTATGTCCAGACTACAGCAACAGCACTGGCGGACTCTGTGGGCCAGGGTACTACTGCC CTCGTGGGTCACCGAGTCCTCTGAATTGTCCTGCAGGAATGTACTGCGAGACACCCGGGCTGGCTGAACC TACTGGTAACTGCTCTGCTGGGTCTTACTGCAACCACACAAGCTTTGAGCCTGATCAGCACGCCTGTCCT CCGGGCTACTACTGCCCCGTTGGGACGGGTGTACCGTTCGCCTGCCCCGCTGGTACGTACAGCAACACCT CGGGCAACGTGGAGCTGGCCGACTGCCTCAACTGCACATCCGGACAGTACTGTGAAGGTGAAGGCAATGC CGAACCGGACAATGACTGCGATCCTGGCTACTACTGTCCCGGCGGCCAAACCTCGTCCAACCCGGTTGGT CTGGAGTGTCCGGAGGGTCACTACTGCCCCGTGGGATCTCATGACCCAATCCTGTGTGAGAACGGAACGT ACCAGGCCAACACGAAACAGCCTTCTTGTGACCAGTGTCCGGGCGGATACTATTGCGATCCAACAGAATC TGGACCAGTCATCACCCCTGTGACTTGCCCTAGGGGGTACTTCTGCCCCCCTGGCACTGGATTATCCACT ACCAATGCCTGCCCAGAGGGTACGTTTGGTGACAGCGATGGTTACGATGATCTGGAAGACTGCGTACAGT GCACGGCAGGCTCGTACTGTGAGGAGCCAGCGTTGTCCACACCGACCGGGGAGTGCTATGCAGGGTTCTA TTGCACTAGCGGGGCATCCAGCCCAACGCCTTATAATGACACGGTCGATGCTGACAACAGTTCATTCACC GGCAATGATGTCTGCCCCAAGGGTTACTACTGCACCAACGGGACCGACTACCCTGAGCCCTGCCCGCCTG GGACATTCTCAAACAACGTTCGGGTGACTGAGGAGGAGGACTGCGATCCGTGTCCCGCTGGAAAATACTG TAATCTTCAAGCTTTCATCAGGGTTAATGAGGCGCCGGACTGCGATGATGGCTACGTCTGTACAGGTGGA AGTACAACCGGTTCCCCCTCCAGCGACAGCGGGATGGGCTACCCTTGCCCGGCCGGTCACTACTGCCCCT CGGGGGTGACCAGTGAGCTGGGTTGCCCCATAGGAACGTATCAACCTGATGAAGGCCAGGCTAACTGTAC CACCTGTCCCGACGGTCTGATGTGCTTGTACACCAATATGACCATGCCAGAGCCGTGTAAATCAGGTCAC TACTGTGTCTTTGGTGAGCAGCTGCCCATCCCTTGTCCTCCTGGCCGATTTAACAACATCACTGGTATCA GCTCAGAGGAGGAGTGTCCATACTGTGTGTCTGGGCAGTACTGCGCTGGCTATGGTAACGTCTGGCCCAC AGGACCATGCACTGCAGGTTACCACTGCCAGGGTGGAGCTGTGGATCAGGTCCCAACGGCCACCTCAGAG TTTTCTGAGAACGGACTTTGTCCCGTCGGCCATTACTGCGACGAGGGCACACCCGCTCCTGAGCAGTGCC CTTCAGGAACCATCCGGTATACCGAGGGTGCGGCGGGCGTCGACGACTGCCTGCCCTGCCCTGGTGGCTT CTACTGCGATACTCCGGGCAAGACCAACGCAACGGCAGAATGCACGGCCGGATTCTACTGCCCGGCCAAC GAGACCATCACTGCCCCGACCCCGCAGGAATTCCTGTGTCCAGTCGGTCACTACTGTCCCGAGGGTAGTC CAGCCCCTGTACCCTGTGGGGTTGGTAACTACCAGCCGGACATGGGAAGGGATTCTTGCATTGCTTGCCA GGCCGGGTACTACTGCCAGAGCTCTCTGTTCCCGGATCCGAAGCCGTGCCCAGCTTATAACTATTGTCCA GCAGCTACACAGACACCGATCCCTTGCCCTGATGGAACTTTCACGTATCCGAACGACACAGGCTTGCAAT CAGAGTATGAGTGCTTGCCCTGTCTTGCTGGGCAGTACTGCCGAGGAGGCCAGCTCCAGGGTGACTGCTC CCAGGGGTACCTGTGCTTGAGTACCAGCTCAGAGTATACACCCAACGGGACCTTCCCCTCCTTGTACGAC GTCGACAAGACCCAGTGCGAGGCCAACACCACCTGTGCCGGTCCCTGCCCCCTCAGCCACTACTGCCCTG AGGGAACAACAGATGCGATCCCCTGCAGGAACTTCACCTATAGGGCCGATTACTTTGGGAGTGTGGAGGA AGACTGCTATCCCTGTGTTGCTGGGAGCTACTGTCTTGAAGGCGACCCTACCCTCTACCCATGCCCCCTG GGCCACTACTGCCTGGAGGGGCTCGCACCCATCGTGTGCCCTCTCTATCATTATCGAGACGTTGAGGGAG CTCCGAGCATGCAGGACTGCTTCAACTGTGAGCCTGGCTACTTCTGTAACGAAACAGGAATGACTAACTA CACCTACTACCCATGCCCCGTTGGTCACTACTGTGAGGAAGCCACGACTGAACCAGAGCCATGCACCGGA GGACGCATGAGCACCACCGAGGCTCGCGTCAGCAACGAACACTGCCCTCTATGTACGCCGGGATACTACT GTCCCAACGATACTATCAACGTCCATGGCATACCGTGTCGACCAACGTATGAGTGCCCTGAAGGAGCTGC AATTGAAGTGACATGCCGAGGAGGATACTACTGCGAAGGAGTGACTGGGCAACCCCCTATCTGTCCAGGA GGCTATTACTGCCCCATCGGATCATTTGCACCAACCATGTGTTTCTACCCGAAATACTGTCCAGAGGGTA CTGAAGTACCCCTTGCGTGTCCCCTAGGCTACAAGGCAGTCAATCATAACGAACTACGTAGCGACGATGC AGGATCTTGCCGTATCTGCCCTGCGGGTACCTATGGCAACCACACGGAGCGTTTCTACTGCGCTGACTGC CCCGAGGGATACTACTGCCCCGAAGGTACTGGCAACCCTCAAGACTACCCGTGCGAAGAAGGCTACTACT GCCCGGCCAATTCCTCGGCACCGACACCCTGCCCACCGGGAACGTTCGGAAACCAGGTCCAAGCGTACAG CGAAGATCAGTGCGTGGACTGCCCCACCAACACCTATAACAACTTGGGTGCTCAGATAGCCTGTAGGCCA TGTGGAAGTGCCTCAAGTGCTGAACCAGGAAGCGCCATGTGCACCTGCAATGGCCTCTACCGATCCTTCC AGATCCTTGATGGATCCTGCGAATGCCAGTCGGGTTACGTCTTCTACGACGAGACCGATCAGGAACAGCA GGAGGGCAACGGCGACGGTGACTGCCAGCCGAGGGTCGACACCCGGTGCGAGCTCTCGCAGACCCGTGAT GCGAGCACCCGCGAGTGTGTGGACCCGGCAGAGGCGGACTGTTCGGTGGCGTGTACTCTTGGTAGCGGTG GAACGTACAACGTAGATCTTGGAAAGTGCGAATGTAATCAGTACACCACCGTGGCGGAAATTTGTGATTC GAACTGCACGGCTAACATGCCGACGACCATGGCTTCTGTCGATGTCAATGGCGTCCTAAGTTTGAGCTCC ACTGATGGATATGGTAACACTATAACCAAGTCCTTGTCAGGAACCATCGGACCCAACGTTCATACAACGG GTCAGAAGAAAACTGAGATGTGTGAAACGTCTCCCACTGGTACCACCTGTGTTATTCCCAAAGATGTGAC CGATGTCGATGCTTTTACATCAGAAGATTCCACAGTGATTGTGACCACGCCGTTGTACACCACTTCTGAC TCCTACACCACGATGAACTACTCCATTGGAGCCACCAACTCATCCCGACGTCGCCTCCTCTCCACCCAGT CAACCACCGGGGTGAGCGGTATCGAGAACCCGCTGGTGTGCCTTGAGCTTGAAGACCTCATTCTCTTCCG TGTCAACATCGACCACAATAACCGCTCCCTCAGCAACTACCCCGTCTACCAGAAGGACCACTTGTTCAAC ACCAACCCGACCTTCGACTACAGCGAGTTCCGCGACCTGCGCTTCTACATCCTCAACACTAACGTGTCCA TCTCGTTCTTCGCCCATGTCTTCACCGAGCCGGGACGTTATGTGATTGGCGATGCGCAGGAGCCGAGCTG GGAGATCATAGTGGCCGTCCAGGAGCAGGATGTCTCCTGCGATTCCATCAGGATCGGACCGTCCAACCCG GCTAACCTGGTCCTGCAGGACGTCAGCAAGACCAACAACGTCAACGAGGAACCTGACTGGGGTCTCATAA TCGGTATGCTTGCTTTCCTTGGTGCCTGTATCATCATGCTGGTGATCGCTGTCATCGTCTGGAGACCCAT GGATGCCGGCATCTACCCGATGAAGGCCTGGAAACCCAAGTACCGCGCCCTAGGTGCCCCACCCAAGATC CCCACCTATCTCCAATACAAGGATCACGAACGGGAGGAGCTGATTGCAGGTACCACTGTAGCCAGCACCT CGGCTGTTGCTGTGTCAGGGATGGCCTCAGGCAATCCCACACTCCTAGAGGACTTCAATGTGCGGACACT GTATGACAAACTGGAAGATCAGACGCTCTATCTCTCACAGCAGCTGGCAAAGCAGCAGGAAGATCTTCAA GGCTTCTATGAACGCATGTCCAAACAGACAGATGGACTGAAGGATCTCCTGGTCAACTTGGATGTCAACA AGCTGGAACGTCTCTCCGGCCACAGACGAGGGCGCCGTGGTAGTGGGTCAAGCAGTGACAGCAGCAACAA TGGCCAAGATATACGTGTCATGAATTTCTCTTCCAGCCGCACCTATAATTTCTCAGGATCCAACAGGAGG GAACAGGAGCTGATGGAGGCTCTGCAGCTCCTGCTGGAGAAGCTCAACACCGGCAAGATCCCCCTGAGCC AGGAGATGCTCCAGCAGGCAGGTCTGGCTCCAGGTGGATCCATCTCTGGAGGAGGCGTGGTGTCCGGGAA GGGAGCCGCCGTGGACAAGCTCCTGGCCAAGCAGAAGGCGGAGCGCATGCACCTGGACAAGGAGCTGAGG GAGGAGGCGGAGAACGAGATGGAGAAGCTCCTAGCAGACCATGATGAAAAGCGTGCCTTGCTGAGCAGGC AGATGTCCGAGAACCTCCAAGCCAAGCTGGAGCAGGCCACCTCCCAGGAGGAAATTGAAAAGATCATGGC CGAGCATGAGAAGAAGTTGGCCGACGGTCTGGATCAGTTGGACAAGCAGAAGGCCAAGCAGATGCAAGAC TTGACCAGGAGGCTGGCCAACCGACGGCGGGACAGAGAGGGTGCCCTCAAAAGGCAACACAAGGCAGAGG CCGAGCAGCTCGGCATAAAGCTGGAAGATGTCGACGGGACGGACCCAGAGGGAGTCCTCCAGGACCAGGG CCTGGACCTGGACCTTCTGATGGCCGAGGAGAACGCTAGCAGGACCAAGGACCAGACTAACATCACAAAG AACCTAGCCAAGGAACAACAGAAGATGATGTCAGAGAACATGAACACTACCTTGACAAGCCTTGAAGAAA CAGGTATCCTCTCAGAGTCTGATGTGAGCGACCTTAAGGTAGAACTGGAGAGCTTGGAAGACGGACTCAA GAGAAAGCTAGAGAAGAGGAAGTCAGACCTCAACAGGAACATGAAGAACAAGATGGCTGAAAAGAGGAGG AGAAAGACAAAGAAACTTCAGACGCAGCACGATAAGGAACTTGAGAAATGTAACTCCAGCGAGGAGGCTG AGGAGGTGGGGAAGAGACATCAGAAGAGACTGGAGACCATGGAGAAATGGCTTGACAGCGAAGAGAATCA ACTAGCTCAGTCAATTGAGAAGGAGATCGGTAATCGATTGGCCAAGGAACTTCAAGAGGGACATAAGGAG ATCCTTGACAAGCTTGCTGGCTCTCACGGTGTCGACCAAGTCACCCAGCAGCGTCTCCTAGATCAGCTTC GTCGCAACAACGAGGACATCTCCTACGAACTTGGGGTCCAGCGCGAGCAGCAGTCCTCCAACTTCGACGC CAAGATCGCGGCCCGTAGAGGGCGCCGTAAGCAGGACATGCACCGCCTCGCCGAGGAGGAGGCCGCTCAA CGCATCCTGAAGGAGCAGGAGAAGGCGGTCGGGAAGAAGGACGATCGGGGCGGATTCAGCATCATGGGCG TGGCGGAAGACACACCCATCAACATGGCAGAGCTCTCCATGGAAGAGCAGGCTGTGGTCAGGGAGCACCA ACGCGTCCAAGAGGAGATGAAGGAGCGCCACGAGCAGGAGAAGGAGGACCTCCAGAAGAACCTTGGTGAG GGAGCCAAGACCAAGGAAGCCCAAGAGGTCAAGAAGCTGGAGCGGGAGAAGTCCAAGGTCCTGTCGGAGA CCCGGAACAAGCACGCCGCGGAGCTGGCCGCCCGTCAGGACCTCTCCGAAGAGGAGATGAAGCTGTTGCT CCAGACGCATGCCCGAGAGCTGGAAGACATCGAGGATCGCATGGAGAGCGAGAGGACCAGACAGCAGCTG GCGGTGCGGGACAGGCTGGCAGCGCGCAAGAAGAGACTCCTGGAGGACCAGAGGCGGAAGCAGGAGCTGG ATCTTGCCAAGGAGGCGATCGAGCAGAAGAAGGAGCTGAGGGATGTCCACGGGAAGGTGGTGAAGGAAGC AGAGAGGAAGGAGATGGTGGAAGAGATACAGAAAGCTGGAGGACAGAACACGGACAAAGTCATCAAAGAG GTTCTTGCCAAACGCCAAGCCCAGGAAGCCAGGGGCCTCGATGCCCAATTTGCTGCTGAACGTCGCATCG CCGTCGAGGGTGCTCTCGACAAGCTCCACGACAAGTACAACGCCAAACGCGACTCCATGCTCCAGAGGCA CGAGAAGGAACTTGCAGATCTGGCCAAGGAAGACCTGACGTCGGACCAACGGCAGCAACGCAAGGCTGCA CTCCTCAACAAGCAGCAGATTGAGCTGAACAAGCTGGAGCGGGAGCTGGCGGACGAGAAGAAGGGCATTG AGAAGGGCGCCCTCAGCGACTGGGAGCTCCGATACGCCCGAGCCAAGCTCCAGATGAAGGAGAGACACTA TCAGGAGTATGCGGATGCCTTGAAAGAGTTAAGCTCAGACCAAGACGCTAACCATGTGGTTAATGCCGAA CAGGCCGCCAAGGCAGCTCAAGAGATTGAAGAAGTCAGGCAGAAGCTAGAATCTGAGAGGATGGCCAACG AGGAGAGGATCAGGAAAGAAAGAGAAGACTTTGAGGAGAACGAGAAGAAGAAGATGGCGCGTGAATTGGA AGAATACGAAAGCAAACTAACTGATGAAGAGAAGAAAGATAGAGAGAAGAACGAGAAGAGAATCAATGGT CTCAACAAGAGAAAAGATCAACTCATCAAAGATAAGAAGAAGAAACAAGAGGAGAAACTGTCGGCCATGA AGGATCAAGGAGCAAGCCAGGAGGAGCAAGAGAAGATCATTGCCGAGTTTGAAAGAGATCTTCAGAAGGT CCAAAACCAGATGGATGCTGATAGACTGAGGATGCAGAGTGAACTAGAGGAGAGAATCCGAAAGCGGAAG GAGGAGAGACGAAAGGCCAAGGTAGAACAGGTGCAGGGCCAGGTGGAGGAGAACATCAAGGAGAAAGAGG AGGAGCTCAAGTCAGAGGAAGAGAGGATGAAGGCGGAAGAGGTAATCCTGCTGCGTAACAGTGTAGAATC AGACCACCTCCTTTCGGATGCTCCCGCCCAACAAGAACAAGCCTTCCCGACGGACTACACCATGGCTGCT GCCCTCAGTGATGCAGACTTGGCCAACCTTCTCATGTCATCACCGCTCTACCGCAAGCTGGGCGAGATCA AGCGTACCATGGAGGATGGTACGCTGCCTCAGCGCAAGCGTGGAGAAGAAATCTTCACAGACAAGAACGA CACCGAATGGACAAACGACCAGGAGCTCGTGCCGATCGACCTCAACAAGCTCTCACCCCGCAGCTTCGTG GCTTACCGCTTTGGATGCTTCGTGGTGCGTCTCGTCGCTGTCCACTGCGGCCACAAGCCCGTCTCGCTGC TCATCGCGGAGAAGATCCCCGTCAACGAGAAGCTTGTGAGGAACGCTTACCGGAACTCGTTCCACTACGA TGACCTGAACCATATCCTGTACGTGAGGGAGGAGAGGCTTGGTAGCGTCGGAGAGTTCATGCTCGTTATC ATCCATGCTCTTGCTCACATCAAAGCAGGTGATATGCGAGACGACACCAACGTCGACTTCCTCCGCGAGT TCCACCTTGCGCTATCCGTCGTTTGCAACGATCTCTTCTTCGCCCGCTACCGCAGCAAGAACTCACCGCG GGACACCACCTCTGACCGGGACGGCGAGGGTATCAGCACCCTGGAGGAGCCGCCACTCGACCCCGCCCAG TCCAAGGCCCTCATCGATGCCATGTTTGGTGGAGAGCAGCTGGAGACAGAGAAGGAGGACTCGTTGGAGA GGCTCATGGACGTCAAGCTCCTGCATGGTAGCGATGAGGATGGGGTTCACTTCAACAAGGAGCTCCTCTC AGAGAGGATGAGCTCGTTCAGTAACTTTAGTCTGAACGACAAGATGAGGAACTTCCTTGGAGCAGTAGAA GATAAGATGGCGTTGTCAAGGCAACATGGGAGCAGTGATGATGTTGACAAACGTCTGGCAAAGCTGACTG GAAAGCCAGCAACATCCAAGACTCATCCAGCTATGAAGGGCATGAAAGGCGCCATTGACCAGCTCCCCGA TGAGCTGAGTGGTACCGCACTCTGGCAGAGTGTGTCCCAGGGAGCTACGGCCAAGGAGCGTTCCGGCGGT CGCATCATGCTGGCTGCTAGCANAGCCCAGGATAAGAACCTACAGAAACAATTCCTTATGGACGAAGTCA ACTCACTGACGGAGAAACTTGATAAGCTCAATACCACCTTCGCAGAGCTCAGCACCAAATACGTCAAGAC GCAGGGCACGATCAGCGCCATCTATGAAGAACTACACTCTCAGAAGACACTTGCAGAGGAAGAGAAGGAG AAGCCTGAGGAGAAGGAGAAGCTGATGAAGGACACAACTAAACTTCTGAGCGATGCACAAAACAACCTGA CCGACCTTAATCTCAAACGGGACACTCTCTCCAAGAAGATTGACTCGTACAGCAAGCTACTTGAAGAGAG ACAGCAGCAACTACAAACCCAACTCAGACGCAAAGATGTCAAGAAGAAGTAAAAACTCCCCTGTTATGCA TAATGGAATGGTCACTTAGTCATAGAGTCGTGCGGTATCTGGGTTATTCCATTTAGTCTCTAAGGGATGA TACTCGTATAAGATTTGAAAATATTGACCTGTACAGTAAGTTATTTGTAGAAGGAAAATACATTTTGTAG CAAGTACACTGCCCACTCGGATGTAAGGATTCAATAAGTGAAAACTCCCCTTTATCGCATAATGGAACAG TCCGTTTGTTATAAAGTCATGTACCATATGGGTTATTTCACTGAGTTAAAGAGGGAAGTGCAGTCTGGTG AGAACTGTTTTACAGTTATACATGTAGGCCACAACTGGGGTGCCAATTTAAGAGCATAAGTTTTTGGTTT ATCTTCCACAGAAATCAAATAGAACAGCCGCAATTTTATAACAATGTGTACAATGAGG
???blast.query.upload???
???blast.options???
???blast.eval???
???blast.eval.01???
???blast.eval.10???
???blast.eval.1???
???blast.eval.001???
???blast.eval.minus5???
???blast.eval.minus25???
???blast.eval.minus50???
???blast.eval.minus100???
???blast.numalign???
???blast.numalign.25???
???blast.numalign.1???
???blast.numalign.5???
???blast.numalign.50???
???blast.numalign.100???
???blast.wordsize???
???blast.wordsize.default???
???blast.wordsize.2???
???blast.wordsize.3???
???blast.wordsize.4???
???blast.wordsize.5???
???blast.wordsize.6???
???blast.wordsize.7???
???blast.wordsize.8???
???blast.wordsize.9???
???blast.wordsize.10???
???blast.wordsize.11???
???blast.wordsize.12???
???blast.wordsize.13???
???blast.wordsize.14???
???blast.wordsize.15???
???blast.wordsize.default.message???
???blast.matrix???
???blast.matrix.blosum62???
???blast.matrix.blosum45???
???blast.matrix.blosum80???
???blast.matrix.pam30???
???blast.matrix.pam70???
???blast.gapedalgin???
???blast.true???
???blast.false???
Automatically set to False for sequences longer than 4500 bytes
???blast.filtering???
???blast.on???
???blast.off???
BLAST time is limited to 30 seconds
