BLAST

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Alignment program	blastn - DNA query to DNA database blastp - Protein query to protein database blastx - Translated (6 frames) DNA query to protein database tblastx - Translated (6 frames) DNA query to translated (6 frames) DNA database tblastn - Protein query to DNA (6 frames) DNA database
Database	-- Genomes -- Strongylocentrotus purpuratus 5.0 Genome Strongylocentrotus purpuratus 3.1 Genome Lytechinus variegatus 3.0 Genome Patiria miniata 3.0 Genome Acanthaster planci 1.0 Genome Lytechinus pictus 3.0 Genome Lytechinus pictus 2.1 Genome Asterias rubens 1.3 Genome Anneissia japonica 1.0 Genome -- mRNA -- Echinoderm mRNA -- CDS -- Echinoderm CDS -- Protein -- Echinoderm Protein
FASTA query sequence	>XM_030973404.1 PREDICTED: Strongylocentrotus purpuratus trichohyalin (LOC580810), mRNA TGAAAAGTTGTATCGTAAACAAGGATTTCAGAACAAGAAGTTAGGCGTGGCAAAACTACCCAAAATTGGC TATTTGCAGGAGGAAATCATCTTAATCGTGCCACTGCTGCATAAAGGAGCACTCCTTGTCATAATGAATC TTCCCCGATGACATCACAATGGCCTCATAAATAGACAGTGCCCTCTACCAGCCATGGTGGAGCTAAAGGT CTGGGTGGACGGGTTCCCGCGAGTGGTGTGCGGGATCAACGAATTCACGACCTGCCAGGAAGTAGTCGTT GCTCTGGCCCAGTCGACGGGTCAGGTCGGGCGCTTCAACCTCTTCGAGAAATCTCCAAAGACAGAGCGTA TCCTGCACCCGCAGGAGAGCCCTTACCGTGCCTTAGAGAAATGGGGTCCCATGTCCAAGAACGTCCGCTA CTACCTCCAACGGAAAGGAACAACGCCGTTTAGTCAGACGCCGCACAGCAGTAGGCCGTCCTCCGTGACG AGCCTCAACCTGTCTAGTCCTGAAGAGCGCTTCATGACCTTGGCTGATTTCAGTGTTGACGGTGATGATG ACTATTTGAGAGTGGCGGCGTATCAGGGGTCGACGAGTGGGGGCGGGAAGCATGGTGCCTTCCAAAGACT AGAGCTAGAAAGACACGCCCAGCTGAGGAAGGAGGAAGAAGAACGGAGGATGCAGGAGGAGGAGAGGAAT AGGGAGATGGAGAGAGATCAAGGTGTTGAGAAGATGAAGGAGATTGAAAGGGAGAGATTTATGGACCATA ATAGAGAGATAGGACGGCAGCATGCGTTAGCCATCGAGAAGGAACAACAAAGGAAGAGAGAGAGGTGGAA GGAGGAGGCAAAGGTGAGAGGGGTGGAGGAGGGGGTGAAGATAAGGGAAATTGAACGTTTGAAAGATGCA GAGAAACAAAGAGAACTTGAACAACAGAGGCAACAGTACCTGAGAGAGCAGGAAGAAAAGGAACGTAAGA TTCGACATTTGGAGGAGATGATGAGAGAAAAGGATAGACAGAAGGAGGATGAGAAGAAGAATGAACTTGA GAGATTGCAAGAGAGGCAAAGAGAAGAACAGAGGGCTAAGGAGGAGGAGATAGCACAATTGAAAGAAGAG GCGAAGAGGAAAGAGTTGGAACGTCAGAAGGAAGAAGAGAAGAGGAAAGAAGCAGAGAAGAAAAAAGAGG CGGAAAGAATAGCAGCCTTGGAGCGCGAGAAGGAGTGGGAGGCTGAGAGGAATAGGTTACAAAGAGAAGC AAAAGCTTTTGAAGAACAGAAGAAGATGGAAGAAGGAAAGAGGAAGGAATTGGAAAAGCAGAGAGAGGAG GATAGGCAAAGAGAAGTGCAGAAGGAAGAGGAGCGAAAGCTAGAGATAGAAAGAGTTCGGAGAGAAACTG AGATAAGGGAAGAGGAGCGTATAGCGGAGCACGAGATTGCACGTCAAATAGAAATACAGAAGCAGAGAGA TATAGCAAGAGAAAAGAGGAAAATAGAAGAGGTATTAAGGAAAAAAGAGATTGAGCATCAAAAAGCACTC CAAAGACATAAAGATGAAGAACTTAAAAGAGAAAAGTTAAGACTTCAGGAAGCGGAATGGGAGAAAGTCA GGATGGCAGAGAAGGAGAGACAGACGGTGGTACAAAGGCAGAAGGAGGAGCGCGAGAATGAACAACAAAA ACAGGAGGAGATAGAAAAGGAGAAAAAGAGACTTGAAGAAGAATTAAAGAAGAGAGAGAATGAAAGGCTC AAGGCCTTGGAGCTTGAGAAGGCTAGGTTAGCGGAGGTCGCCAGGAGAACGGAGGAAGAGCGACTGAAGG AGATCGAGAGACAGAAGGAGTTGGAGATTCTAAGGGCGGAGGAGCTGCAGAAGAAGGAGAAGGCAAGAAG GGAGCGCATCGAGATGGAGAAGGTCAAAATTTTGGAGGCCAGAAGACAGAAAGAAGTCGCCAGGAAAAAA GACGAAGATCAGAAGCGACAGAAGGAGAAGGAGAAGATCAACGAGCTGAACCAGATTGTTAGCGTGCAAC AGGAGCGGATTGAGACGCAGATATCACAACTCACCAACATCGAGGAGGCCTGTCTGTCCCTTCAGATGTT TGAGCTGGATCCGGAGTTTGACCAGATCGAGGATAAGATCATCGAGTTTGAGCAGGCGGTCAAGAAGAAC AGGAGCGCTCTCAAGGAGATGGAATTCTGGGAGAACGAGCTGGAGATCGAGCAGCAGCTGTGCGAGAGAC TCATGAAGGAGGTGAAAACGTTGAAGGCGAAGGTACACGAATGTCAGAAACAGACGGAGGTCAAACAAGC GGAGATAGAGGAGATAGATGAAAGGATACGGAATGAGGAAGAGAGGATCGAAAGAGAGGGAGATGAGCAA GCAAAACAAGATCTTCATGCTGTCCAGGTTGAACTGACGGTGTTCCAGAAGGAGCTCGAAGGCTACGCAG ACAGTCTCCAAGAAGTCAACGAGGACATCCAGGAAGTGGAGAGACTCCTAGAGAAGAAGCAAAAGGAGAT TAAGAAGCTCGACAAGGAGATTAAGGAAGAAGAAGAGAGGAAAAGACAGGAAGAAGAAAGGAAGAGAGAA GAAGAAGAGAGGTTGAGAGCAGAAGAAGAAAGGAAATTTGTTGAAGAGGAAAGGCAGAGAGCGGAAGAAG AAAATAAGAGAGTCGAAGAGGAGAGGAAGAAAAAAGAAGACCAACAGAAAAAGAGAGCGAAGGAAGAAAG GAGGAGATTGGAAGAAGAAAGGAGAGTGGAGGAAGAAAGAAAGAAAGAAGAAATGAAGAAGGCAGAAGAA GAACGGAAGAGAGCAGAGGATGAGAGGAAGGAAGAGGAGAACAAGGTAGCAGAAGAGAAGAGAAAGGAGG CGGCTCAGAAGGAGAAAGAGGATCAAGAGAAGGATGGAGAGAAGAAGAAACTTGGAGAACTCATCAAAAA TCCAACTCCTGGAAACAATCCTGCGCGTAAGATGCTCAAAAGCCCTAGAGACCTCTTCAAGCAAGCACCG AGCTCGGACAACCCTGAAGGCGTCTTCGTTTAAAGCCCCTCTGCACTACTTATAGCTACTTGCGCCCTCT ATATAGCAAACAATGCCTAATCGGTGCCTGTTGGTCCTCCATGATCCTCTTTTTCTTATGGTCATTGAGA GCTGATTTGATGAGATAACCACCTGTCAGTGACCTTGCAGGGCGGTCTAATCCCCCTGGCCAAACTAGTG CAGACTGGCTTTAAACACCCCTGCTATCAGAATGGAACAACCCACCTTTTGTACGATGCTTCCAGCTCAT TTGGCTAGATGACACCCCCATTATTAGAATGGATTGATCCGTCTGTCGTAGGTTGCCCTCAGCTCATTTG GTTAGATGGCACCCCTATTATCAGAATAGAACAACCCACCTTTTTGTAGGATGCTTCCAGCTCATCTGGC TAGATGACACGCCTATTGTTAGAATGGATTGATCCACCTGTTGCAAGATGCTTCCAGCTCATTTGGCTAG ATAACACCCCTATTATCAGAATGGATTTATCCACCTGTTGCAGGTTGCCTCCAGCCTATTGGTTAGATAG TAGTGGTGATTATTTTACCTTACTGCTAAGACGACACCCCTATTATCAGAATGGACCTTTCCTCCAGTTG GCCGGTAATCGGGTCAATATAAGGGGGGTCCATATTTTTTTGGTGTCGGGAATCATTTTTATCTAATCAG GAAAAAAGAATGTTCATTCCACACAGAAATGAGCCTTATTTATGGTATCTGTTTGTAGATGGAATGCATG TTGCCGAATGACCATGTAAACTTAAAGCAGATGCATTAAATTTCAAGAATTATATTTAAATTTTTGTATA AATAAGCTGCATATGTTATAGACCATAGGGACTGCTGAGAGCCAGAAGGGTGTTATTCTTGGATTGCTGT CCTCCAAAAGATTCTGTGATGGAATATAAATTGTCCTATCAACCCATACTGTACATATAGCAGTGCAGTA TCTCCTTTTAGAGAGGTTTTTGCATTATCTTTTGATGTACAATGCTAGGATTTACACATTATACATGAGC AGAAAAAGCAAAAATCACAGAAAAAGTTTAGTCTAAGCTGTTGACGAATACGGTAAGAATGATTTAATTG TAACTTATCATCGAAATTACTAAATTGGCAACACGTAAAATATTAAATCACTATTTTTGTGTACAAATTG GCAATTTTGCAGTTTAATATTACAATTTACATTTACATTGGTTATATTAAAATGCAAACTTTTTTTCAAA CTATGTACAGGTAGGAGTGTAAAATTAGAATAATTGTTGCCAATTTGACGAGAAAAACTGATGAATTTAG AAATCTTTGAACTGCTATGAGTTAAAACACAGAGTTATTACTCCCTTTTGCTTCTCAGTATGTAATATAT ATCTTTGTGACAAGGCTTTAGTTCTTGATGCGAAACTCAGAAGAACAAGTGAAATCTTGCCATGTTTTAT GTCAAAATGTTTAAGAAAAATGCTATATGAATGCATTTGATTATTAGACAAATGCATTGAATGAATTTGG GTTGCTTGCACATGAGGCAGTAAATTACCTTCCAAAAACTTGGCCTTTGAAAGTAATATACCGTAATAAT CCTAGATTCCTATCACTCTTTTTTCCTTCTCCTTTCTCACAGCACAAGCATGTGCTGTATAAGAGATTCA AAATTTTGCTTCGAGTGATTCCAAATTTGTATTCATGAGATTTCAGATGTTTGCAGTGTAAAATTAGTAG GTTTGGCTTGCAATAAAATATGGTTAAGCCTACATCATTACTGGTACTTCTTTGTACCATGTGTCAACAT TGCTATTGAGTAGCATCCAAGTGGTTTTATTTCACCATAAGGGCTTTTCAGTGAAGTGATTCACTGCGTC ATGTGCAAACATCACACAGCATTGAATACCTACATGTACATGTATGTATAGATAAATGTATTTATGGTGT GGTATACAATATTCATGTAAAATTTATTAGCTTAGCAGTATCGGTGCATGGGATATTATATATTATTATC TTATATTTTGGGGAAGAGGGACATAAGATTTACATGTATGTTATGAATTTCCTTGTCATTTTATTTTTAA TGATTATATTGTTTGAAGATCAAATTTAGTACTTTGACAATCGATTCAGATGTATTACAACTTTGAAAGG AGTTTGATATTCCAGGTAAAGAAAAAGCAACTGTTCATGTAAGTAGTGAGATATGTTATTTTAAGTGTGC AAAGAATTTTGAGGACTTCCAATCGATCTGGATCAATTGCGGCTATTTGATCTGGTTGGAATTAGTGCTT TATATGTGGTTCTTCAAGAGAAAAATACCGTATGATGGTTCTGACTGTTACTTAAGGCAAACCAGATTGT CCATTGCGAGTACTTAATTTCAAAGTCAGCCATTCATGACATGTACTGTAGCTGTAGGCCTATACATGAG CCATCCCATGTTTGTCAAGGAATGTTTTGATTTTGTATCACTTTATTGTCAATCTTCAATAAAGAACCAG TATAAACATAAAAGAATTGCAGTCAGAGACATATAATAAGAATATTTACTGTAATTTGTAAAACTCATGA AAACGTAATCATTGTGAAGTACATGTATAACACATATGCAGGGGATGTATTATTGATGGACCAAATTAAA TATGTACAACTGTACATGTACATATATACTTTGAACCCACCACACATTGTACAATCTCCTGCGATCCGAT TTTCAAAAAATGGCAAATCTACAGTGTATTAGATACATACTGGTAAACATTATCTTCGTAAGTTAGGTTC TAGATCATAAGAATACTAACACTTTGAATTTGATTGCATGTAAGGTTTCTAGTCAGAATAAAAGCCGAAT CAGGTTCAAATCGTAGACTATCACACGACTCATCTGGGCTCATTTCACAAAACTTGTCATGAGTGACAAA TGACAGTTTTTGTTATAAGCTACTGAAATCCTTGTTTCTGATTGGTGATCAGCAGATTTGTCACTGATTT TTGTCATTTGTCATTGAAAAAAAGGCTTTGTGAAACGGGTCCCTGATGTTATTGTGATTCTGAGCCAATT TAGGTTTTCCATCTTCAAGGAAGTTTGAAATCCATTAAATTTAGATTTCATTAATCATAAGACTATTTAG ATCCTCGATCATTCACATTTGAATCATTTGAATGTTTATATGAAATGCTA
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E value	0.1 (Default) 10 1 0.001 1e-5 1e-25 1e-50 1e-100
Number of alignments	25 1 5 50 100
Word size	Default 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Default = 11 for blastn, 3 for all others.
Matrix	BLOSUM62 (Default) BLOSUM45 BLOSUM80 PAM30 PAM70
Gapped alignment	True False Automatically set to False for sequences longer than 4500 bytes
Filtering	On Off
	BLAST time is limited to 30 seconds