BLAST

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Blast program	blastn - DNA query to DNA database blastp - Protein query to protein database blastx - Translated (6 frames) DNA query to protein database tblastx - Translated (6 frames) DNA query to translated (6 frames) DNA database tblastn - Protein query to DNA (6 frames) DNA database
Database	-- Genomes -- Strongylocentrotus purpuratus 5.0 Genome Strongylocentrotus purpuratus 3.1 Genome Lytechinus variegatus 3.0 Genome Patiria miniata 3.0 Genome Acanthaster planci 1.0 Genome Lytechinus pictus 3.0 Genome Lytechinus pictus 2.1 Genome Asterias rubens 1.3 Genome Anneissia japonica 1.0 Genome -- mRNA -- Echinoderm mRNA -- CDS -- Echinoderm CDS -- Protein -- Echinoderm Protein
FASTA query sequence	>JT096280.1 TSA: Strongylocentrotus purpuratus WHL22.566454.0 mRNA sequence GCTTATTATCAGCTTGACTGGATGTCTGATGATAATGACTTGCATGGCCCGGAGATCGAGCGAGGTTTTG ATTGTAAAACCTGCTCGAGCAGCGCATATATATCATCACAATACGACGAGAGATAGTATTTAATGAGATG ATTGGTGCAAGGAGTGGATCGCACTGTCGCGTCGGCTGCTGTGTGTCATACGAAAACAAGGAGTGATCTA CTTACATGAAAATCAACCATCACTGTGGAGAGAGCTATTCCATTGTGATCAAACTCGCTCCTGATCCTCT CCTGTAAATGTAGCGATGTGCTTGGACCATCTTGCCTCTACCATCAAACCGAGGACCTAGTTAAATTTAG TTTGTGGACAAAGAGCCACTCAGATTGCAGGAGAGAACCCGCAGGAGGAACAGCGTGACGATAGCTGACT TCAAGATACAACCCGTAAACTCCTCTTTAAGAAGTAGAACAATTTTCTCCTTCAGATGAATCGAAAATAT GTGACGTTGCCTAATTCAAGACGTAAGTTTCACAGACTCCGTCATCTAGGAGGAACTATGGCGCCGAACA AAGCTAAGCATCAGACATTGGGAACGCAGAATTATGCATTTGAGGCGGACGAAGCGGTTGATTCCCATCG GGACAACTCGTCTACAGTTCCAGAGGCCAAGTCACCAATGACTCCAAGCAACACCAAAGGCAATGTCGGC GGCAATGTAGGGATATTGGGCAACTCGGTCGAACTCTTGGTGTCCGCAGCAGATCATCTTCGACCGTGCG CGGATTGTCTAAAGCGCCAATTCGACGCGGCTCATGAAGCGACAACATTAGTCTCGCAGTCCAGGCTTTT TAACATTCAAAAACTGACCGAGGCGTTGTTACCGATTGTAGAGGTGACTGAACGACTGCAGAAGAGTCTG AGTGCTGTCCAGGATACAAAACCGGAGAAAATCTTCACGCCAGTTCTGATCAATACACTTACAAAGGAGT CTACTAAAACGGAAAGAATCTCTAAAAGTCTTTCAACGGACAAAGCGGACGCCACGCGACTAGATCAAGA TCTACGGGAGATTCGAACCGCCATCCTGGGGTGTATCGAGGCGTTTGAACCGGTGCGACAAGCCGTCTTC CGCCCTGACCGCCGAGGCGACCACGACTACGACCGCGTTGGAAACCAAACTCGCGAGTATCTATGGACAT TGGATATCTATTGGCAGGGATTACGTCAGGCGAGAGAGGCTCTACCAAACGAGCCGAAACTCCCGCATGT TGCTACCGACACAGAAAATGAACCTCTTACAAGAAGTTCCGGTGCTGAGCTGCTTGAATCGGCATCAAAA CGGTACTGGCCGGTGTAGAAATACTTCCATTAATATCAGTAAAGATATTTCGATGATATAATGATCGAAG TATGCTTGCATAAATCTTGCTATAGATAATATCTGATGAGCTTAATCAACTGCTGGACTGGCGATTTTCA TCTCAGTCGAAGGAGACTCTCCCGAACGAGCCGAAACTGCCGTAGCAGTTTGAAACAGTATCAAAATGGT ACCGGCTGGTGTTGAGATACTTATATTGTCAATGAAGAAACTGTTACCTTTATTTAAATACATAAGATTG TGCAGATCGAGCTGATACATCTACTGTCGAGAATCGGGCAAGTCGGCAATGGCTTTCCCGAACGAGCCGA AACTTCCGTATGTGGACGACCTTGAAAAATAAACCCTTTACAAAAGGGGCATTATTTTGAAATGCGGTAT ATTCGTACATTAAAATTATTAAGCCCATACGCCAAAGTTTCAAAGCAATCTTATGGGGAAAGGTCATGTT CATATGCAGCCACCGAGTTATAGAATTATTTGCCGAAATACCTTATAGATATAGTAACTTCTAACAAGTT CAGAAGTAATTTAAAACATACTTATTTAACACTTTGTAAGATCTAATAATTATGAACCCAGTTTACCATT TCCACTCCTTCTGTATTCTGTTCTGTAAGGCACATGATAATAGTCATGCAAAAATAATATTATACCTATG TATTATGCGCTGTACACAAATGTTTAACTATTATTATTATTATTAATCTGAACATTCTTCCATTATTTTT GTTCGATATCAGGAAAGTACACATTCCTTGAAATGTAATGTGCGTCGGCCATGGATGCATGCATAAACCA TGGTGGACGTATGATGGTCTTGTGGAAAATTAACCAAGCAGTGGATGATCTGTGGATCACAAGGAACGAG GATCGAATCCCTGCCACGGTACTAGTTTCCTCCGGCAAGACATTAATCTACATTTACCTCACCACACTCG AGCCAGGTGAAGCAAGTGGGTTCCGGTGGATAATTATTTCGTGAATGCTACAAAGCCCTTTTTAGAGTAG CTATAGCTAGGCTAAGCTGGGGATAACTTTGATGATTCAATCAAAAGCTCCTTGGAAGGGTATATGGACT ATCATTTTGTGATATGCGGTATACAAGAGTAAAAA
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E value	0.1 (Default) 10 1 0.001 1e-5 1e-25 1e-50 1e-100
Number of alignments	25 1 5 50 100
Word size	Default 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Default = 11 for blastn, 3 for all others.
Matrix	BLOSUM62 (Default) BLOSUM45 BLOSUM80 PAM30 PAM70
Gapped alignment	True False Automatically set to False for sequences longer than 4500 bytes
Filtering	On Off
	BLAST time is limited to 30 seconds