Genome Assembly 1.0

What's New

Pmin_1.0 is the latest (as of Apr 11, 2012) assembly of the genome of Patiria Miniata. The assembly tools CABOG (Celera Assembler), Newbler, ATLAS-Link, and ATLAS-GapFill were used to assemble a combination of 454 reads (fragment and 2.5kb insert paired ends;~15x coverage) and Illumina reads (300bp insert and 2.5kb insert paired ends;~70x coverage).

Introduction

This information is for the first release (Pmin_1.0) of the draft genome sequence of the Patiria miniata . This is a draft sequence and may contain errors so users should exercise caution.Typical errors in draft genome sequences include misassemblies of repeated sequences, collapses of repeated regions, and unmerged overlaps(e.g. due to polymorphisms) creating artificial duplications.

With a goal of solving the polymorphism issues of the data while maintaining the sequence continuity, The Pmin_1.0 assembly was generated in the following steps:

1) 454 reads were assembled by CABOG using settings less strignent than the default (unitigger=bog utgErrorRate=0.03 ovlErrorRate=0.08 cnsErrorRate=0.08 cgwErrorRate=0.14 doExtendClearRanges=0)

2) Both contig and degenerate sequences from the previous step were chopped into fake reads with ~11x coverage (500bp long; 460bp overlap; 80bp minimal length) for ctgs and 8x coverage(450bp long; 400bp overlap; 80bp minimal length) for degs. The fake reads were then assembled by Newbler with the option of -large.

3) Both 454 and iIlumina pair end reads were mapped to the contigs from the previous step. We used BLAT to map the 454 data and bwa(aln+samse) to map the Illumina data, both with the default options. Based on the mapping locations of the paired ends, contigs were then ordered and oriented into scaffolds using ATLAS-Link.

4) ATLAS-GapFill was then used to assemble the reads locally in an attempt to fill the gaps among the contigs within the scaffolds.This final step produced 770.5Mb sequences with contig N50 size of 9.5kb and
scaffold N50 size of 50.3kb.

Conditions for use

These data are made available before scientific publication with the following understanding:

- The data may be freely downloaded, used in analyses, and repackaged in databases.

- Users are free to use the data in scientific papers analyzing particular genes and regions if the providers of this data (Baylor College of Medicine Human Genome Sequencing Center) are properly acknowledged.
Please cite the BCM-HGSC web site or publications from BCM-HGSC referring to the genome sequence.

- The BCM-HGSC plans to publish the assembly and genomic annotation of the dataset, including large-scale identification of regions of evolutionary conservation and other features.

- This is in accordance with, and with the understandings in the Fort Lauderdale meeting discussing Community Resource Projects and the resulting NHGRI policy statement (http://www.genome.gov/page.cfm?pageID=10506537).

- Any redistribution of the data should carry this notice.

Description of files

There are 2 directories.

I. Contigs/ directory

This directory has 2 files for assembled contigs in the genome, there is
no chromosome assignment for the contigs in Pmin_1.0.

Pmin_1.0.20120411.contigs.agp (agp file)
Pmin_1.0.20120411.contigs.fa (fasta file)

The Pmin_1.0.20120411.contigs.agp file describes the positions and
orientations of the contigs in the group. It takes the standard NCBI
format.

II. LinearScaffolds/ directory

This directory has 1 file

Pmin_1.0.20120411.linear.fa

The sequences are linearized scaffolds where the gaps between adjacent
contigs within a scaffold are filled with 'N's and the captured gap size
is estimated from the clone insert size.

Sequence statistics

Scaffolds/Contigs Number N50(kb) Bases(Mb) Gap(Mb)
All Scaffolds 60,336 50.3 811.6 41.1
All Contigs 181,436 9.5 770.5 N/A

History

Pmin_1.0 (Apr, 2012) This release was the first assembly of the
Patiria Miniata genome.